Mulberry (Morus alba L) species is native to northern China and is widely cultivated various Asian countries such as Japan, Iran, etc. Morus alba was traditionally used in Chinese medicinal remedy for ailments of bronchitis, insomnia, constipation and inflammatory. The research was planned to appraisal nootropic activity of aqueous and ethanol extracts of Morus alba using conditioned avoidance response in rat and estimation of acetyl cholinesterase activity by Ellmanʾs method in rats. Conditioned avoidance response was evaluated by using the Perspex chamber apparatus. Animals were treated with scopolamine butyl bromide (1mg/kg bw, i.p) thirty minutes before foot shock to produce amnesia. Animals were trained to jump on the pole to avoid shock with receiving daily oral dose of aqueous and ethanol extracts of Morus alba at dose of 200 and 400 mg/kg body weight one hour before the induction of foot shock. The esterase activity was measured by providing an acetyl thiocholine which cause to release thiocholine as result of cleaving by AChE. Thiocholine reduced 5,5’-dithiobis- (2-nitrobenzoic acid) (DTNB) to thionitrobenzoic acid which absorbed light at 412 nm. The groups treated with aqueous and ethanolic extracts of Morus alba were found to nootropic activity by reversing the scopolamine induced amnesia. Acetyl cholinesterase inhibitory activity of extracts of Morus alba were performed the supportive nootropic activity by enhancing the cognitive function.
Mulberry (Morus alba L) species is native to northern China and is widely cultivated various Asian countries such as Japan, Iran, etc. Morus alba was traditionally used in Chinese medicinal remedy for ailments of bronchitis, insomnia, constipation and inflammatory. The research was planned to appraisal nootropic activity of aqueous and ethanol extracts of Morus alba using conditioned avoidance response in rat and estimation of acetyl cholinesterase activity by Ellmanʾs method in rats. Conditioned avoidance response was evaluated by using the Perspex chamber apparatus. Animals were treated with scopolamine butyl bromide (1mg/kg bw, i.p) thirty minutes before foot shock to produce amnesia. Animals were trained to jump on the pole to avoid shock with receiving daily oral dose of aqueous and ethanol extracts of Morus alba at dose of 200 and 400 mg/kg body weight one hour before the induction of foot shock. The esterase activity was measured by providing an acetyl thiocholine which cause to release thiocholine as result of cleaving by AChE. Thiocholine reduced 5,5’-dithiobis- (2-nitrobenzoic acid) (DTNB) to thionitrobenzoic acid which absorbed light at 412 nm. The groups treated with aqueous and ethanolic extracts of Morus alba were found to nootropic activity by reversing the scopolamine induced amnesia. Acetyl cholinesterase inhibitory activity of extracts of Morus alba were performed the supportive nootropic activity by enhancing the cognitive function.