Leishmaniasis is an emerging zoonotic disease of global importance. Dogs serve as natural reservoirs for various Leishmania species in urban areas. However, with the use of repellents, vectors may seek secondary reservoirs such as domestic cats. The clinical manifestation of leishmaniasis in felines differs from dogs by presenting predominantly cutaneous lesions, which can be easily confused with other conditions including fungal diseases and neoplasms. Given the disease’s global significance, coupled with the increasing number of affected species and non-specific clinical presentations, this study aims to report the diagnosis, cytological and histopathological features of cutaneous and ocular lesions caused by Leishmania sp. infection in a domestic cat from an endemic area in Ipatinga, Minas Gerais, Brazil. The animal was presented with an acute wound at the base of the left ear canal and chemosis in both eyes. The feline underwent hematological, biochemical, and cytological examinations, which confirmed the diagnosis of leishmaniasis through identification of amastigote forms of the protozoan. Following diagnosis, the owner elected for euthanasia. Tissue samples from the ocular conjunctiva and skin were submitted for histopathological examination, which confirmed both leishmaniasis and the presence of squamous cell carcinoma These findings highlight the need to include cats in the epidemiological chain of leishmaniasis, demonstrating the importance of considering this disease as differential for both dermatological and ocular lesions diagnosis in felines.