In this research, attempts were made to study the effects of cow dung and cow bone char mixture for bioremediation of crude oil-polluted soil. This process of remediation was conducted ex-situ using an optimization technique termed three-level design with two factors -𝟑𝟑 𝟐𝟐 design factorial. The first-order kinetics was also employed in studying the kinetics of degradation with the observed correlation of determination (𝐑𝐑 𝟐𝟐 ) between (0.726 -0.969).The optimization technique shows also that the optimal mix range between 20 -38% for cow dung and 35 -50% for bone char. In terms of mass, this translates to an optimal mix of 30-57g of cow dung and 52.5 -75gof bone char for every 2kg of soil having a 4% crude oil contamination relative to the total mass of the mixture.