In establishing a viable strategy for resource mobilization for biodiversity, determining the level of expenditure incurred is a vitally important factor. Inasmuch as, it allows to guide decision-making and evaluate the effectiveness in the allocation of resources, and to know the contributions of different actors. Likewise, a management document would be available to the financing entities, and inputs that contribute to optimizing the management of resources, identifying emerging needs, and promoting participation in the processes aimed at the financial sustainability of development and conservation. Consequently, an analysis of the behavior of the biodiversity expenditures of key institutions in the Guantánamo province is carried out, using theoretical, empirical and mathematical statistical methods, for the conceptual theoretical foundation, the analysis of the established policies and the characterization of the main organizations and institutions that affect the problem addressed, and the proposal for improvement.