global-journal-of-science-frontier-research-g-bio-tech-genetics Global Journal of Science Frontier Research - G: Bio-Tech & Genetics 0975-5896 2249-4626 Global Journals Publishing Group Incorporated 59375 DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers UkaChiedozie Joel AwomukwuDaniel NyananyoBio Louis NIGERIA, Federal University 07 06 2015 15 G1 1 12

Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were extracted, purified, PCR amplified and sequenced using plastid Ribulose -1,5 bisphosphate carboxylase large chain (rbcL a) and Maturase K (matK) genetic marker to identify unknown Phyllanthus species. The plastid region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on Gen Bank and Bold Systems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and Bold Systems using both rbcL and matK gene regions while P. amarus Schum and Thonn and P. urinaria Linn. were clearly compatible with other works. P. muellerianus (O. Ktze) Excel was only compatible with other works using matK gene region but none for rbcL.

 Phyllanthus species DNA extraction PCR amplification nucleotide sequences BLAST Bold Systems rbcL matk Barcoding. Full Text

DNA of five species of Phyllanthus in Nigeria namely, P. amarus Schum and Thonn, P. urinaria Linn., P. odontadenius Mull-Arg., P. niruroides Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were extracted, purified, PCR amplified and sequenced using plastid Ribulose -1,5 bisphosphate carboxylase large chain (rbcL a) and Maturase K (matK) genetic marker to identify unknown Phyllanthus species. The plastid region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on Gen Bank and Bold Systems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and Bold Systems using both rbcL and matK gene regions while P. amarus Schum and Thonn and P. urinaria Linn. were clearly compatible with other works. P. muellerianus (O. Ktze) Excel was only compatible with other works using matK gene region but none for rbcL.