Aims: This study is to isolate and characterized the bacterial isolates associated with pneumonia in Sudanese camels using different methods and test the sensitivity of some isolates to antibiotics. Study design: A total of 800 affected lung samples were collected from 400 camels (Camelus dromedarius ) of different ages, seasons and part of the Sudan. Place and Duration of Study: The study was undertaken in the laboratories of the Veterinary Research Institute, Ministry of animal resources and fisheries during 2009 to 2013. Methodology: The bacterial isolates were characterized by three different methods: the conventional, Api kits and automated Vitek 2 Compact System. Fourteen of the Gram negative and 11 of the Gram positive bacterial isolates were tested for sensitivity to antibiotics by the Vitek 2 Compact using a sensitivity card which contained 20-21 antibiotics.