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global-journal-of-medical-research-c-microbiology-pathology

Global Journal of Medical Research - C: Microbiology & Pathology

0975-5888 2249-4618

Global Journals Publishing Group Incorporated

10.34257/GJMRC60057

60057

Study of Î±-Amylase Inhibitory Activity of Fractions Purified by Chromatographic Methods of the Endophytic Fungus Penicillium Brevicaule Alba-CC200

Iqbol

Mukhammedov

Tashkhan

Gulyamova

Dilaram

Ruzieva

Saodat

Nasmetova

Sarvinoz

Turdimuhammadova

UZBEKISTAN, Andijan State University 23 C2 15 20

In this study, the antidiabetic activity of purified fractions of the endophytic fungus Penicillium brevicaule alba-CC200 isolated from the plant Celosia cristata was investigated. Antidiabetic activity was evaluated by inhibition of α-amylase enzyme. The secondary metabolites of P. brevicaule alba-CC200 were partially purified by column chromatography and obtained fraction II inhibited α-amylase by 91.2%. High inhibitory activity was observed in fraction II and was determined to be cardiac glycosides and terpenoids by thin layer chromatography (TLC) and qualitative analysis. This requires a more precise study of the purified fractions using high-precision chromatographic methods.

a-amylase a-glucosidase column chromatography TLC cardiac glycosides terpenoids.

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In this study, the antidiabetic activity of purified fractions of the endophytic fungus Penicillium brevicaule alba-CC200 isolated from the plant Celosia cristata was investigated. Antidiabetic activity was evaluated by inhibition of Î±-amylase enzyme. The secondary metabolites of P. brevicaule alba-CC200 were partially purified by column chromatography and obtained fraction II inhibited Î±-amylase by 91.2%. High inhibitory activity was observed in fraction II and was determined to be cardiac glycosides and terpenoids by thin layer chromatography (TLC) and qualitative analysis. This requires a more precise study of the purified fractions using high-precision chromatographic methods.