Equine infectious anemia (IEA) is a worldwide distribution disease caused by a lentivirus associated withthe presence of transmitting vectors of the gender Tabanus and equines by producing anemiaandconsequently hypoxia that produces a multi-organ deterioration. The presence of AIE in the working equine of San Andrés Itzapa, Chimaltenango was evaluated. This in order to assess the presence of the disease since this group of equines is part of the food security of the community. 100 blood samples were analyzed using the gel agar immunodifusion test. The presence of the disease was found in 8% of the sampled animals. The prevalence was higher in females than in males. The meanage of the horses sampled was 5 years. The mean bodyconditionof the study population was 2. The presence of the disease in the study population should be evaluated and considered as a starting point for the implementation of control and prevention measuresgiven the vector spread of the disease. -age and the impacton the community due to the zootechnical function of the sampled animals.