Background: When root canal infections are concerned, the causative factor is primarily the microbial inhabitation, which leads to the spread of infection. The literature evidence supports the aspect that frequent usage of antibiotics induces resistance and threatens the effectiveness of the treatment. So, the current issue that is faced globally is the development of resistant microbial species. To combat this interest, towards the usage of newer herbal horizons is increased. Materials and Methods: The antibacterial effect of Ethanolic Leaf Extract of Morinda Citrifolia was investigated against Enterococcus Faecalis (E. Faecalis). Agar well diffusion and broth dilution methods were used to determine the Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: The MIC of Ethanolic Extract of Morinda Citrifolia extract was found to be 250 μg/ml, and the MBC was 400 μg/ml