Background: University undergraduates are at an age when experimentation with sex is prevalent. A significant number of young people still indulge in high risk sexual behavior. Hence, profiling safer sex would be important for the future health of this group. More so an improved knowledge among this group could act as a step down during peer interactions with other non-medical undergraduates. Aim: This study was designed to assess the knowledge and practice of safer sex among Medical undergraduate students and to know to what extent their practice impacts on their health as regards contraction of sexually transmitted infections and having an unwanted pregnancy. Subjects and methods: In this cross-sectional study, three hundred and fifty (350) Medical undergraduate students were interviewed using pretested semi-structured self-administered questionnaires to assess their knowledge and practice of safer sex.