In spite of the fact that substantial advancement have been made in the remedy and control of cancer progression, remarkable inadequacy for improvement remain. Natural therapy can diminish adverse effect of chemotherapy. Currently over 60% of the drugs are derived in one or other way from natural source including plant, marine organism and micro-organism. The present investigation was concerned with pharmacological potential of honey bee venom and ethanol extract of Zingiber officinale Roscoe towards anticancer activity. The cytotoxic potency of combination of honey bee venom and ethanol extract of Zingiber officinale Roscoe was evaluated on cultured cancer cells Hep-2 by sulphoradamine B assay (IC50=53 μg/ml) and moreover, it was invested by brine shrimp lethality assay (IC50=48.31 μg/ml), tryphan blue exclusion assay (IC50=37.49 μg/ml), and MTT assay. MTT assay exhibited that combination of honey bee venom and ethanol extract of Zingiber officinale Roscoe has more cytotoxicity potency towards human breast adenocarcinoma cells (MCF-7) than normal Chinese hamster lung fibroblast cells (V79). In EAC ascites model significantly ascending of life span with restoring of haematological parameters and additionally, in DLA solid tumour model crucial decline in the tumour weight and tumour volume was observed as compared to control.