Background: Factor V Leiden is the most common inherited form of thrombophilia with 3 to 8 percent European and American ancestry carrying one copy of the factor V Leiden mutation in each cell, and about 1 in 5,000 people have two copies of the mutation. The mutation is less common in Asian, Hispanic and African populations. Aim: This study was carried out to know the incidence of Factor V mutation in various regions of India. Methods: The presence of factor V mutation was determined by PCR- RFLP from patients suspected of thromboembolic etiology from medical, surgical, obstetrics and neonatology departments. Results: Analysis of 94 patients with coagulation disorders, factor V mutation was seen in 20 cases. Discussion: Although many reports from India indicate the absence of this mutation or the presence of homozygous mutation in a low level we report about 26 percent of the cases having the homozygous mutation which is the first for India.