Different protocols are used at transport layer of the OSI model to transmit real time applications (i.e., video and voice). In this paper, two networks were analyzed in which the Transmission Control Protocol (TCP) and the User Datagram Protocol (UDP) were used for transportation. The purpose of this study is to evaluate the performance of TCP and UDP in real time transmissions for the parameters: Traffic Received, End-to-End Delay, and variation in delay (Jitter). At the receiver side, an algorithm called Chunk-based Jitter Management (CJM) is applied on the buffer, which divides the packets into small size chunks and then plays them out. The evaluation of these transmission protocols has been done in OPNET. The graphs show the results in which the CJM algorithm performs well in end-to-end delay, jitter in voice packets, and voice packets receiving.