The Internet technology has enabled companies to create a new market space that facilitates electronic interactions among multiple buyers and sellers. It is believed that the perceived benefits of e-commerce have a great impact on e-marketplaces usage. However, research shows that supply chain management (SCM) influenced by ecommerce. The purpose of this study is to investigate the relationship between B2B e-commerce benefits, supply chain management and e-marketplace usage. The proposed model was tested on managers of companies in different Industries in Amman – Jordan. Structural equation modeling technique was employed using AMOS 7.0 to verify the reliability and validity of the multi-item scales and to test the hypothesized relationships. Finding indicates that the perceived benefits of e-commerce are significant in explaining the variation in emarketplace usage. Results also revealed that B2B ecommerce has a strong and positive direct and indirect effect on supply chain management. It has also been found that there is a significant positive impact of E-marketplace usage on supply chain management. The findings contribute to understanding the relationships between B2B e-commerce benefits, supply chain management and e-marketplace usage, provide critical implications for managers; and highlight directions for future research.