Introduction: Neuropathic pain is one of the reasons for intervention in diabetic patients, and photobiomodulation (PBM) is a non-pharmacological therapeutic alternative, which has been increasingly used in recent years, with modulatory actions on cellular energy metabolism. Objective: To evaluate the acute effect generated by photobiomodulation with light emitting diode (LED) on pain in individuals with neuropathy resulting from type 2 diabetes mellitus. Methods: The study consisted of the evaluation of the signs and symptoms of neuropathic pain (Douleur Neuropathique Questionnarie – DN4) and blood flow in the posterior and dorsal tibial arteries (Doppler ultrasound) after the application of PBM for three consecutive days in 58 patients.