In the Brazilian Northeast, the state of Piauí exhibits a lacunose chiropteran fauna in terms of diversity. Thus, knowledge about the ecology of these animals, highlighted by the occurrence of significant zoonoses, remains abstruse. The study aimed to update the geographical distribution of Diphylla ecaudata in the Brazilian Northeast, associating it with new foraging approaches and records of the rabies virus in the region. For the construction of this work, bibliographic searches were conducted in journals and digital platforms reporting the occurrence of Diphylla ecaudata bats in Piauí. The research included field activities in the municipalities of Picos, Pedro II, and Milton Brandão, setting up mist nets at dusk and dismantling them before dawn in areas suspected of the presence of these animals. The captures followed specific protocols cited in specialized literature under authorization 69264-7 SISBio, 002/CEUAIFPI/2021, and registration AF78746 in SISGEN.