Aflatoxin Risk in Dairy Production: Assessment of Dairy Cattle Feed Contamination Level by Aspergillus Flavus and A. Parasiticus in both Conventional and Traditional Dairies

Gabriel Kehinde Omeiza

Aflatoxin Risk in Dairy Production: Assessment of Dairy Cattle Feed Contamination Level by Aspergillus Flavus and A. Parasiticus in both Conventional and Traditional Dairies

Gabriel Kehinde Omeiza * §

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University of Abuja

Aflatoxin Risk in Dairy Production: Assessment of Dairy Cattle Feed Contamination Level by Aspergillus Flavus and A. Parasiticus in both Conventional and Traditional Dairies

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Abstract

Contamination of dairy cattle feeds by aflatoxigenic Aspergillus group, poses public health challenges as a result of high chances of aflatoxicosis. In this study, therefore, dairy cattle feed samples (n=144) collected from both conventional and traditional dairies were examined for the presence of aflatoxigenic strains of Aspergillus flavus and Aspergillus parasiticus using microbiological and molecular techniques. Fungal Colonial counts (CFU) were determined, and the mean CFU/g of the feed samples was 3.8 ± 0.47. A Significant number of the feeds, 86 (59.7%) showed positive contamination level, out of which 55.8% and 18.6% (representing 33.3% and 8.3% of the total dairy cattle feed samples collected) were contaminations due to Aspergillus flavus and Aspergillus parasiticus respectively. All the 64 isolates of the A. flavus and A. parasiticus were examined for aflatoxin producing abilities under a long UV light (365 nm). Aflatoxin production levels were quantitatively determined using ELISA technique and 16 isolates representing 25.0% of the total isolates; in the ratio of 3:1 respectively, showed a varied level of production of aflatoxins.

References

29 Cites in Article

Reference Format

M Abarca,M Bragulat,M Bruguera,F Cabanes (1988). Comparison of some screening methods for aflatoxigenic moulds.
H Abbas,R Zablotowicz,H Bruns,C Abel (2006). Biocontrol of aflatoxin in corn by inoculation with non-aflatoxigenic Aspergillus flavus isolates.
Samir Abbès,Jalila Salah-Abbès,Hakimeh Sharafi,Rania Jebali,Kambiz Noghabi,Ridha Oueslati (2013). Ability ofLactobacillus rhamnosusGAF01 to remove AFM1in vitroand to counteract AFM1immunotoxicityin vivo.
M Ajala,B Nwagu,E Otchere (2004). Socio-Economics Of Free-Range Poultry Production Among Agropastoral Women In Giwa Local Government Area Of Kaduna State, Nigeria.
O Atanda,M Ogunrinu,F Olorunfemi (2011). A neutral red desiccated coconut agar for rapid detection of aflatoxigenic fungi and visual determination of aflatoxins.
S Bandh,A Kamili,A Bashir (2012). Identification of some Aspergillus species isolated from Dal Lake, Kashmir by traditional approach of morphological observation and culture.
(2008). Canback Global Income Distribution Database (C-GIDD).
P Chang,C Skory,J Linz (1992). Cloning of a gene associated with aflatoxin B1 biosynthesis in Aspergillus parasiticus.
G Criseo,A Bagnara,G Bisignano (2001). Differentiation of aflatoxin-producing and non-producing strains of Aspergillus flavus group.
Joe Dorner,Richard Cole (2002). Effect of application of nontoxigenic strains of Aspergillus flavus and A. parasiticus on subsequent aflatoxin contamination of peanuts in storage.
J Dorner,R Cole,D Wicklow (1999). Aflatoxin reduction in corn through field application of competitive fungi.
Rolf Geisen (1996). Multiplex Polymerase Chain Reaction for the Detection of Potential Aflatoxin and Sterigmatocystin Producing Fungi.
Amaia González-Salgado,Teresa González-Jaén,Covadonga Vázquez,Belén Patiño (2008). Highly sensitive PCR-based detection method specific for Aspergillus flavus in wheat flour.
M Hedayati,A Pasqualotto,P Warn,P Bowyer,D Denning (2007). Aspergillus flavus: human pathogen, allergen and mycotoxin producer.
S Ibrahim,M Rahma (2009). Isolation and indentification of fungi associated with date fruits (Phoenix dactylifera, Linn) sold at Bayero University, Kano, Nigeria.
G James,S Natalie (2001). Unknown Title.
Davise Larone (1995). Medically Important Fungi: A Guide to Identification, Fifth Edition.
R Latha,H Manonmani,E Rati (2008). Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic Aspergilli.
Biing-Hui Liu,Fun Chu (1998). Regulation ofaflRand Its Product, AflR, Associated with Aflatoxin Biosynthesis.
(2004). Research at Health Department's Wadsworth Center Holds Promise for Alzheimer's, Parkinson's, Huntington's Diseases.
G Naidoo,A Forbes,C Paul,D White,T Rocheford (2002). Resistance to Aspergillus Ear Rot and Aflatoxin Accumulation in Maize F Hybrids.
M Rashid,S Khalil,N Ayub,W Ahmed,A Khan (2008). Characterization of Aspergillus flavus and Aspergillus parasiticus isolates of stored wheat grains in to aflatoxigenics and non-aflatoxigenics.
Mam Romo,Cartagena,Mcr,Ferri,Efr,G Fernández (1986). Minimal moisture content for growth and aflatoxin production by Aspergillus parasiticus in mixed feeds.
M Saghai-Maroof,K Soliman,R Jorgensen,R Allard (1984). Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics..
D Somashekar,E Rati,A Chandrashekar (2004). PCR-restriction fragment length analysis of aflR gene for differentiation and detection of Aspergillus flavus and Aspergillus parasiticus in maize.
M Tvrtković (2006). Aflatoxin M 1.
I Udom,C Ezekiel,S Fapohunda,Okoye,Zsc,C Kalu (2012). Incidence of Aspergillus section flavus and concentration of aflatoxin in feed concentrates for cattle in Jos, Nigeria.
T White,T Bruns,S Lee,J ; Taylor,M Innis,Yang (1990). Amplification and direct sequencing of fungal RNA genes for philogenetics. In: Detection of aflatoxinproducing molds in Korean fermented foods and grains by multiplex PCR.
J G Willem,P Melchers,P Verweij,H Van-Den,Alex,V Ben,E Jacomina,A Hoogkamp,K Jacquesf,Gmm (1994). General Primer-Mediated PCR For Detection Of Aspergillus Species.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Gabriel Kehinde Omeiza. 2019. "Aflatoxin Risk in Dairy Production: Assessment of Dairy Cattle Feed Contamination Level by Aspergillus Flavus and A. Parasiticus in both Conventional and Traditional Dairies". Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 19 (GJMR Volume 19 Issue G2).

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GJMR Volume 19 Issue G2

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

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Classification

GJMR-G Classification NLMC Code: WA 360

Submission ReceivedDecember 6, 2018
Peer Review Double Blind
Handling Editor
Accepted January 5, 2019
Published January 15, 2019

Version of record

v1.2

Issue date

April 30, 2019

Language

English

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Total Score: 141

Country: Nigeria

Subject: Global Journal of Medical Research

Authors: Gabriel Kehinde Omeiza (PhD/Dr. count: 0)

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