Comparative Studies on the Antioxidant enzymes in Bone Marrow Precursor cells and mature Rat Polymorphonuclear Leukocytes

Ms Swechha Mishra

Comparative Studies on the Antioxidant enzymes in Bone Marrow Precursor cells and mature Rat Polymorphonuclear Leukocytes

Ms Swechha Mishra * § PhD

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§ Indian Institute of Information Technology Allahabad

Comparative Studies on the Antioxidant enzymes in Bone Marrow Precursor cells and mature Rat Polymorphonuclear Leukocytes

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Abstract

Continuous blood cell production throughout the lifetime of an individual is ensured by the hematopoietic stem cells (HSCs), which are bone marrow cells that possess extensive self renewal capacity and ability to differentiate to all the blood cell lineages. The redox status of the bone marrow stroma is an important factor determining whether the HSCs would differentiate or remain quiescent. Lower levels of ROS (reactive oxygen species) are required to maintain HSC quiescence, whereas higher ROS levels drive the HSCs out of quiescence and reduces their self renewal capacity. The ROS levels in the bone marrow should therefore be regulated to maintain the self renewal capacity of HSCs. Endogenous antioxidants play an important role in regulation of the ROS levels. The present study was therefore undertaken to examine the changes in the cellular detoxifying defences against superoxide and H2O2 i.e. superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx), in the precursor cells from the rat bone marrow and in the rat blood polymorphonuclear leukocytes. An increase in the Catalase and GPx activities in the precursor cells over the differentiated cells was seen. However, the SOD levels in the differentiated cells were greater than in the precursor cells. The levels of Nitric Oxide (NO)-an antioxidant and a regulator of superoxide generation-in the precursor cells were also found to be greater than those in the differentiated cells. The results of the present study thus indicate an up regulation of the endogenous antioxidants in hematopoietic precursor cells, which might be related to the maintenance of their self-renewal capacity.

References

28 Cites in Article

Reference Format

1. J Karlsson (1997). Introduction to Nutraology and Radical Formation. Antioxidants and Exercise, 1-143.
2. Florian Muller (2000). The nature and mechanism of superoxide production by the electron transport chain: Its relevance to aging. AGE, 23(4), 227-253.
3. Carroll Cross,Barry Halliwell,Edward Borish,William Pryor,Bruce Ames,Robert Saul,Joe Mccord,Denham Harman (1987). Oxygen Radicals and Human Disease. Annals of Internal Medicine, 107(4), 526-545.
4. B Aims,M Shinegava,T Hagen (1993). Oxidants, antioxidants and the degenerative diseases. Proc Natl Acad Sci, 90, 7915-7922.
5. Shugo Ueda,Hiroshi Masutani,Hajime Nakamura,Toru Tanaka,Masaya Ueno,Junji Yodoi (2002). Redox Control of Cell Death. Antioxidants & Redox Signaling, 4(3), 405-414.
6. E Boon,A Downs,D Marcey (2007). Catalase Structural Tutorial Text. Boyum. Scand J Immunol, 5, 9-15.
7. Jack Cowland,Niels Borregaard (1999). Isolation of neutrophil precursors from bone marrow for biochemical and transcriptional analysis. Journal of Immunological Methods, 232(1-2), 191-200.
8. S Greenberg,J Xie,X Zhao,O Jie,T Giles (1996). An in vivo cytokine and endotoxinindependent pathway for induction of nitric oxide synthase II mRNA, enzyme, and nitrate/nitrite in alveolar macrophages. Biochem Biophys Res Commun, 227, 160-161.
9. Stephen Pember,Raymond Shapira,Joseph Kinkade (1983). Multiple forms of myeloperoxidase from human neutrophilic granulocytes: Evidence for differences in compartmentalization, enzymatic activity, and subunit structure. Archives of Biochemistry and Biophysics, 221(2), 391-403.
10. P Kakkar,B Das,P Viswanathan (1984). A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys, 2, 130-132.
11. Hugo Aebi (1984). [13] Catalase in vitro. Methods in Enzymology, 105, 121-126.
12. Sunita Sheokand,Anita Kumari,Veena Sawhney (2008). Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chickpea plants. Physiology and Molecular Biology of Plants, 14(4), 355-362.
13. Stephen Pember,Raymond Shapira,Joseph Kinkade (1983). Multiple forms of myeloperoxidase from human neutrophilic granulocytes: Evidence for differences in compartmentalization, enzymatic activity, and subunit structure. Archives of Biochemistry and Biophysics, 221(2), 391-403.
14. Hans-Georg Kopp,Scott Avecilla,Andrea Hooper,Shahin Rafii (2005). The Bone Marrow Vascular Niche: Home of HSC Differentiation and Mobilization. Physiology, 20(5), 349-356.
15. Toshio Suda,Fumio Arai,Shigeto Shimmura (2005). Regulation of Stem Cells in the Niche. Cornea, 24(8), S12-S17.
16. Zhixing Li,Linheng Li (2006). Understanding hematopoietic stem-cell microenvironments. Trends in Biochemical Sciences, 31(10), 589-595.
17. G Saretzki,L Armstrong,A Leake,M Lako,Von Zglinicki,T (2004). Stress defense in murine embryonic stem cells is superior to that of various differentiated murine cells. Stem Cells, 22, 962-971.
18. Safak Yalcin,Xin Zhang,Julia Luciano,Sathish Mungamuri,Dragan Marinkovic,Cécile Vercherat,Abby Sarkar,Marcos Grisotto,Reshma Taneja,Saghi Ghaffari (2008). Foxo3 Is Essential for the Regulation of Ataxia Telangiectasia Mutated and Oxidative Stress-mediated Homeostasis of Hematopoietic Stem Cells. Journal of Biological Chemistry, 283(37), 25692-25705.
19. J Breton-Gorius,J Guichard (1975). Fine structural and cytochemical identification of microperoxisomes in developing human erythrocytic cells. Am J Pathol, 79, 523.
20. A Marcus,D Zucker-Franklin,L Safier,H Ullman (1966). Studies on human platelet granules and membranes. Journal of Clinical Investigation, 45(1), 14-28.
21. M Denton,N Spencer,Hrv Arnstein (1975). Biochemical and enzymic changes during erythrocyte differentiation. The significance of the final cell division. Biochemical Journal, 146(1), 205-211.
22. J Breton-Gorius,W Vainchenker (1986). Expression of platelet proteins during the in vitro and in vivo differentiation of megakaryocytes and morphological aspects of their maturation. Semin Hematol, 23, 43.
23. G Magrinat,S Mason,P Shami,J Weinberg (1992). Nitric oxide modulation of human leukemia cell differentiation and gene expression. Blood, 80(8), 1880-1884.
24. K Lin,G Austin (2002). Functional activity of three distinct myeloperoxidase (MPO) promoters in human myeloid cells. Leukemia, 16(6), 1143-1153.
25. M Yim,P Chock,E Stadtman (1993). Enzyme function of copper,zinc superoxide dismutase as a free radical generator. J Biol Chem, 268, 4099-4105.
26. M Peled-Kamar,J Lotem,I Wirguin,L Weiner,A Hermalin,Y Groner (1997). Oxidative stress mediates impairment of muscle function in transgenic mice with elevated level of wild-type Cu/Zn superoxide dismutase. Proceedings of the National Academy of Sciences, 94(8), 3883-3887.
27. Katalin Nagy,Gabriella Pásti,László Bene,Imre Nagy (1993). Induction of Granulocytic Maturation in HL-60 Human Leukemia Cells by Free Radicals: A Hypothesis of Cell Differentiation Involving Hydroxyl Radicals. Free Radical Research Communications, 19(1), 1-15.
28. Elevter Russanov,Margarita Kirkova,Milka Setchenska,Henry Arnstein (1981). Enzymes of oxygen metabolism during erythrocyte differentiation. Bioscience Reports, 1(12), 927-931.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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Not applicable for this article.

How to Cite This Article

Ms Ms Swechha Mishra. 2018. "Comparative Studies on the Antioxidant enzymes in Bone Marrow Precursor cells and mature Rat Polymorphonuclear Leukocytes". Global Journal of Medical Research - K: Interdisciplinary GJMR-K Volume 18 (GJMR Volume 18 Issue K3).

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GJMR Volume 18 Issue K3

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

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Classification

GJMR-K Classification NLMC Code: WH 200

Submission ReceivedDecember 15, 2017
Peer Review Double Blind
Handling Editor
Accepted January 5, 2018
Published May 31, 2018

Version of record

v1.2

Issue date

May 31, 2018

Language

English

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Article Matrices

Total Score: 104

Country: India

Subject: Global Journal of Medical Research - K: Interdisciplinary

Authors: Ms Swechha Mishra, Dr Sonia Chadha, Sayali mukherjee, Sangeeta Singh (PhD/Dr. count: 0)

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