Detection of Antibodies against Borrelia Burgdorferi in Periparturient Cows and Calves during the First Year Old by Indirect Enzyme-Linked Immunosorbent Assays (iELISA)

Jenevaldo Barbosa da Silva

Detection of Antibodies against Borrelia Burgdorferi in Periparturient Cows and Calves during the First Year Old by Indirect Enzyme-Linked Immunosorbent Assays (iELISA)

Jenevaldo Barbosa da Silva * §

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carlauzedo §

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§ Faculdade de CiAncias AgrArias e VeterinAria (FCAV/U...

Detection of Antibodies against Borrelia Burgdorferi in Periparturient Cows and Calves during the First Year Old by Indirect Enzyme-Linked Immunosorbent Assays (iELISA)

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Abstract

The aim of this study was to understand the dynamics of circulating antibodies against Borrelia burgdorferi in cows during the peripartum and in their calves during the first year of life, using an indirect enzyme-linked immunosorbent assay (iELISA). Sera from twenty cows and their calves were tested by an iELISA for detection of homologous antibodies against B. burgdorferi. The test showed 93.33% sensitivity and 86.66% specificity. At pre-partum, birth and post-partum, cows showed seropositivities of 90%, 30% and 86%, respectively. However, only 50% of the calves were seropositive after colostrum ingestion. The calves’ seropositivity over their first year ranged from 0% in the second and third months to 64% at 12 months. Therefore this region should be considered an enzootic instability area.

References

21 Cites in Article

Reference Format

1. A Alves,R Madureira,R Silva,F Corrêa,R Botteon (2004). Freqüência de anticorpos contra Borrelia burgdorferi em cães na região metropolitana do Rio de Janeiro. Pesq. Vet. Bras, 24, 203-206.
2. A Barbour,G Maupin,G Teltow,C Carter,J Piesman (1996). Identification of an uncultivable Borrelia species in the hard tick Amblyomma americanum: possible agent of a Lyme disease-like illness. J. Infect. Dis, 173, 403-409.
3. C Bennett (1995). Ticks and Lyme disease. Adv. Parasitol, 36, 343-405.
4. F Corrêa,R Teixeira,C Oliveira,J Barbosa,A Fonseca (2012). Detection of anti-Borrelia burgdorferi antibodies in buffaloes (Bubalus bubalis) in the state of Pará, Brazil. Rev. Bras. Parasitol. Vet, 21, 338-341.
5. Volker Fingerle,Ulrike Schulte-Spechtel,Eva Ruzic-Sabljic,Sarah Leonhard,Heidelore Hofmann,Klaus Weber,Kurt Pfister,Franc Strle,Bettina Wilske (2008). Epidemiological aspects and molecular characterization of Borrelia burgdorferi s.l. from southern Germany with special respect to the new species Borrelia spielmanii sp. nov. International Journal of Medical Microbiology, 298(3-4), 279-290.
6. A Fonseca,M Ishikawa,C Soares,C Massard,N Yoshinari (1996). Lyme borreliosis serology in cattle in Brazil. Revta. Univ. Rural, Sér. Ciênc. da Vida, 18, 85-89.
7. Andreas Frey,James Di Canzio,David Zurakowski (1998). A statistically defined endpoint titer determination method for immunoassays. Journal of Immunological Methods, 221(1-2), 35-41.
8. K Gallo,A Fonseca,R Madureira,J Barbosa Neto (2009). Freqüência de anticorpos homólogos anti-Borrelia burgdorferi em eqüinos na mesorregião metropolitana de Belém, Estado do Pará. Pesq. Vet. Bras, 29, 229-232.
9. Guedes Junior,D Araújo,F Silva,F Rangel,C Barbosa Neto,J Fonseca,A (2008). Frequency of antibodies to Babesia bigemina, Babesia bovis, Anaplasma marginale, Trypanosoma vivax and Borrelia burgdorferi in cattle from the northeastern region of the state of Pará, Brazil. Rev. Bras. Parasitol. Vet, 17, 105-109.
10. M Ishikawa,A Fonseca,C Soares,C Massard,N Yoshinari (1997). Padronização de ensaio imunoenzimático ELISA indireto para pesquisa de anticorpos IgG anti-Borrelia burgdorferi em bovinos. R. bras. Med. vet, 19, 166-168.
11. R Machado,H Montassier,A Pinto,E Lemos,M Machado,I Valadão,L Barci,E Malheiros (1997). An enzymelinked immunosorbent assay (Elisa) for the detection of antibodies against Babesia bovis in cattle. Vet. Parasitol, 71, 17-26.
12. E Mantovani,I Costa,G Gauditano,V Bonoldi,M Higuchi,N Yoshinari (2007). Description of Lyme disease-like syndrome in Brazil: is it a new tick borne disease or Lyme disease variation?. Brazilian Journal of Medical and Biological Research, 40(4), 443-456.
13. E Mantovani,R Marangoni,G Gauditano,V Bonoldi,N Yoshinari (2012). Amplification of the flgE gene provides evidence for the existence of a Brazilian borreliosis. Rev. Inst. Med. trop. S. Paulo, 54, 153-158.
14. G Margos,A Hojgaard,R Lane,M Cornet,V Fingerle,N Rudenko,N Ogden,D Aanensen,D Fish,J Piesman (2010). Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii. Ticks Tick Borne Dis, 1, 151-158.
15. J Parker,K White (1992). Lyme borreliosis and mental disorders: literature review. Medical Sciences, 10(2), 253-274.
16. A Rogers,R Smith,I Kakoma (1999). Serologic cross-reactivity of antibodies against Borrelia theileri, Borrelia burgdorferi and Borrelia coriaceae in cattle. Am. J. Vet. Res, 60, 694-697.
17. I Sasaki,C Davis,B Larson (1976). Production and turnover for IgG1 and IgG2 immunoglobulins in the bovine around parturition. J. Dairy Sci, 59, 2046-2055.
18. U Schulte-Spechtel,G Lehnert,G Lieg,V Fingerle,C Heimer,B Johnson,B Wilske (2003). Significant improvement of the recombinant Borrelia-specific IgG immunoblot test by addition of VlsE and DbpA homologue derived from Borrelia garinii for diagnosis of early neuroborreliosis. J. Clin. Microbiol, 41, 1299-1303.
19. A Stefancikova,G Stepanova,M Derdakova,B Petko,J Kyselova,J Ciganck,L Stronjny,L Cislakova,M Travnicek (2002). Serological evidence for Borrelia burgdorferi infection associated with clinical signs in dairy cattle in Slovakia. Vet. Res. Commun, 26, 601-611.
20. S Wells,A Trent,R Robinson,K Knutson,R Bey (1993). Association between clinical lameness and Borrelia burgdorferi antibody in dairy cows. American Journal of Veterinary Research, 54(3), 398-405.
21. Xiaofeng Yang,Taissia Popova,Martin Goldberg,Michael Norgard (2001). Influence of Cultivation Media on Genetic Regulatory Patterns in Borrelia burgdorferi. Infection and Immunity, 69(6), 4159-4163.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Jenevaldo Barbosa da Silva, adivaldofonseca, carlauzedo, rafaella.medvet. 1970. "Detection of Antibodies against Borrelia Burgdorferi in Periparturient Cows and Calves during the First Year Old by Indirect Enzyme-Linked Immunosorbent Assays (iELISA)". Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 13 (GJSFR Volume 13 Issue D6).

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GJSFR Volume 13 Issue D6

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

antibodies borrelia bovines elisa spirochaetes

Classification

GJSFR-D Classification FOR Code: 030104

060107

Submission ReceivedApril 18, 2013
Peer Review Double Blind
Handling Editor
Accepted May 9, 2013
Published May 24, 2013

Version of record

v1.2

Language

English

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Article Matrices

Total Score: 105

Country: Brazil

Subject: Global Journal of Science Frontier Research - D: Agriculture & Veterinary

Authors: Jenevaldo Barbosa da Silva, Bruna de Azevedo BaAta, Carla Carolina Dias Uzedo Ribeiro, Rafaella CAmara Teixeira, Adivaldo Henrique da Fonseca (PhD/Dr. count: 0)

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