Engineered Bovine Serum Albumin Nanoparticles Incorporated Alpha Amylase Preparation for the Improved Activity

karthi namachu

Engineered Bovine Serum Albumin Nanoparticles Incorporated Alpha Amylase Preparation for the Improved Activity

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Engineered Bovine Serum Albumin Nanoparticles Incorporated Alpha Amylase Preparation for the Improved Activity

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Abstract

In the present study, the improved enzyme activity of alpha amylase encapsulated with bovine serum albumin nanoparticle (BSA) was carried out. Initially. The method for preparation of BSA nanoparticles was optimized with various parameters such as pH, acetone to BSA ratio, cross linking time and concentration of BSA. The optimal pH was found to be 4.5, 10 mg/ml of BSA concentration, acetone to albumin ratio was to be 4.5, cross linking time of 3hrs which gives the higher yield of BSA nanoparticles. The Nano-enzyme conjugate was prepared using the optimized conditions. The encapsulation of α-Amylase Enzyme with BSA Nanoparticle was done based on hydrophobic ion pairing (HIP) complexation. And the Scanning electron micrographs of BSA nanoparticle encapsulated enzyme at the targeted size of 100nm. Distinct changes in the kinetic parameters of nano albumin encapsulated α-amylase was observed. Enzyme activity of nano encapsulated alpha amylase was found to be maximum at the pH of 8.0, temperature 80ºC and 4.5mg/ml of substrate concentration.

References

11 Cites in Article

Reference Format

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

karthi namachu. 2014. "Engineered Bovine Serum Albumin Nanoparticles Incorporated Alpha Amylase Preparation for the Improved Activity". Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 14 (GJSFR Volume 14 Issue G1).

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GJSFR Volume 14 Issue G1

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

amylase enzyme activity nano albumin nanosphere

Classification

GJSFR-G Classification FOR Code: 100303

Submission ReceivedFebruary 25, 2014
Peer Review Double Blind
Handling Editor
Accepted March 25, 2014
Published April 9, 2014

Version of record

v1.2

Issue date

May 7, 2014

Language

English

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Article Matrices

Total Score: 103

Country: India

Subject: Global Journal of Science Frontier Research - G: Bio-Tech & Genetics

Authors: S. Karthick Raja Namasivayam, Sasikumar, Shaleesha Stanley (PhD/Dr. count: 0)

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Publish Date: 2014 04, Wed

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