Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise using a Chemiluminescence Assay

Savignone C, Palacios A

Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise using a Chemiluminescence Assay

Savignone C, Palacios A * PhD

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Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise using a Chemiluminescence Assay

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Abstract

The present investigation was carried out to determine the presence of oxidative alterations in the horses erythrocyte membrane during a high intensity exercise test. The degree of peroxidation was estimated by chemiluminescence using a suspension of lysed erythrocytes incubated with t-butyl hydroperoxide (t-BHP). Differences were observed in the total values of chemiluminescence throughout the exercise routine, with higher values of light emission obtained with the animal at rest in relation to those observed during and after exercise.The conclusions of this study are the existence of changes in the erythrocyte membranes of the horses exposed to physical exertion, probably associated with the release of ROS caused by the exercise and that the determination of chemiluminescence in suspension of lysates erythrocyte is a sensitive assay applied to detect the existence of oxidative stress associated to physical exercise.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

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How to Cite This Article

Savignone C, Palacios A. 2017. "Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise using a Chemiluminescence Assay". Global Journal of Medical Research - K: Interdisciplinary GJMR-K Volume 17 (GJMR Volume 17 Issue K1).

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GJMR Volume 17 Issue K1

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

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Classification

GJMR-K Classification NLMC Code: WH 150

Submission ReceivedDecember 16, 2016
Peer Review Double Blind
Handling Editor
Accepted January 1, 2017
Published January 15, 2017

Version of record

v1.2

Issue date

April 1, 2017

Language

English

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Total Score: 142

Country: Argentina

Subject: Global Journal of Medical Research

Authors: Savignone C, Palacios A (PhD/Dr. count: 0)

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