Isolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa

Zahra Zahid Piracha

Isolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa

Zahra Zahid Piracha * §

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Isolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa

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Abstract

Pseudomonas aeruginosa is an opportunistic pathogen, frequently associated with nosocomial infections worldwide. Multiple drug resistance has been reported in previous studies against pathogenic P. aeruginosa and the biofilm which makes use of antibiotics futile. Bacteriophages specific for P. aeruginosa can prove to be a new therapeutic approach for controlling infections and biolfilm contamination against this pathogen. The aim of our study was to isolate and partially characterize virulent phage specific for P. aeruginosa from sewage water. Different parameters of which make phages as suitable candidate towards future therapeutics were also investigated. Phages having lytic life cycle, high burst size and thermally more stable proposed themselves as effective therapeutic candidates. In this study, a virulent phage was isolated from sewage water having burst size of 1036 and latent period of 21 minutes. This phage has narrow host range and shows the remarkable thermal resistance and is viable up to 60 ºC.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Zahra Zahid Piracha. 2014. "Isolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa". Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 14 (GJMR Volume 14 Issue C1).

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GJMR Volume 14 Issue C1

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

isolation partial characterization phage pseudomonas aeruginosa

Classification

GJMR-C Classification NLMC Code: WC 330

Submission ReceivedDecember 7, 2013
Peer Review Double Blind
Handling Editor
Accepted January 2, 2014
Published January 15, 2014

Version of record

v1.2

Issue date

March 8, 2014

Language

English

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Article Matrices

Total Score: 144

Country: Pakistan

Subject: Global Journal of Medical Research

Authors: Zahra Zahid Piracha, Umar Saeed, Aqsa Khurshid, Waqas Nasir Chaudhary (PhD/Dr. count: 0)

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