Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice

Marcelo Vidigal Caliari; Fabricio Marcus Silva Oliveira; Bernardo Coelho Horta; Luana Oliveira Prata; Andrezza Fernanda Santiago

Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice

Marcelo Vidigal Caliari * §

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Fabricio Marcus Silva Oliveira

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Bernardo Coelho Horta

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Luana Oliveira Prata

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Andrezza Fernanda Santiago

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Federal University of Minas Gerais. Av. AntAnio Carl...

Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice

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Abstract

Studies have shown that CD1 double negative mice (CD1d -/-) develop larger liver abscesses due to their inability to present amebic antigens to NK T lymphocytes. Therefore, we conducted flow cytometry studies to determine the frequency of NK T, CD4 + T, CD8 + T and B lymphocytes in mice with amebic colitis. The frequency of NK T, CD8 + T and B lymphocytes was reduced in the MLN of mice in the CTRL-CD1 -/-and Eh-CD1 -/-groups compared to the CTRL-WT and Eh-WT groups. There was also a significant decrease in the frequency of B lymphocytes in the spleens of the animals in the Eh-WT group when compared with the CTRL-CD1 -/-, Eh-CD1 -/- and CTRL-WT groups. The results of the flow cytometry analysis highlight the importance of NK T lymphocytes in the immune response of mice to amebic intestinal infection and the importance of CD1 molecules in the activation of T and B lymphocytes.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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How to Cite This Article

Marcelo Vidigal Caliari, Fabricio Marcus Silva Oliveira, Bernardo Coelho Horta, Luana Oliveira Prata, Andrezza Fernanda Santiago. 2015. "Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice". Global Journal of Medical Research - F: Diseases GJMR-F Volume 15 (GJMR Volume 15 Issue F4).

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GJMR Volume 15 Issue F4

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

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Classification

GJMR-F Classification NLMC Code: WC 524

Submission ReceivedApril 15, 2015
Peer Review Double Blind
Handling Editor
Accepted May 5, 2015
Published May 15, 2015

Version of record

v1.2

Issue date

November 2, 2015

Language

English

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Article Matrices

Total Score: 145

Country: Brazil

Subject: Global Journal of Medical Research

Authors: Marcelo Vidigal Caliari, Fabricio Marcus Silva Oliveira, Bernardo Coelho Horta, Luana Oliveira Prata, Andrezza Fernanda Santiago (PhD/Dr. count: 0)

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