Use of NMR to Determine Compatable Solutes in Halophilic Bacteria Isolated from Highly Saline Areas

Reda Hassan Amasha

Use of NMR to Determine Compatable Solutes in Halophilic Bacteria Isolated from Highly Saline Areas

Reda Hassan Amasha * §

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King Abdulaziz University

Use of NMR to Determine Compatable Solutes in Halophilic Bacteria Isolated from Highly Saline Areas

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Abstract

Ten halophilic bacteria (two Gram-negative) belonging to the Halomonadaceae and (eight Gram-positive), belonging to the Bacillaceae, were isolated fromtheRed Sea, Arabian Gulf and Dead Sea using a high salinity medium, followed by identification using 16S rRNA.Four of the isolates were designated on the basis of their tolerance to highsalinity. The isolates respectivlyexhibited 97% homology to Halomonasaquamarina, 97% homology to Sediminibacillus sp., (Red Sea), 94% homology to Halobacillus sp., (Arabian Gulf) and 98% homology to Halobacillusdabanensis(Dead Sea). 1H-NMR spectroscopy was used to determine the osmolytes accumulated by H. aquamarina, Sediminibacillus sp.,Halobacillus sp. and H. dabanensis grown in a saline nutrient medium at varying concentrations of NaCl and a range of organic sources. In the case of H. aquamarina, betaine and ectoine concentrations increased at high salinities. In contrast, betaine was found when casein and peptone were used as nutrient sources, while ectoine was produced in the presence of peptone.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

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Not applicable for this article.

How to Cite This Article

Reda Hassan Amasha. 2019. "Use of NMR to Determine Compatable Solutes in Halophilic Bacteria Isolated from Highly Saline Areas". Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 19 (GJSFR Volume 19 Issue C1).

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

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Classification

GJSFR-C Classification FOR Code: 060599

Submission ReceivedSeptember 22, 2018
Peer Review Double Blind
Handling Editor
Accepted October 20, 2018
Published November 2, 2018

Version of record

v1.2

Issue date

March 7, 2019

Language

English

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Total Score: 165

Country: Saudi Arabia

Subject: Global Journal of Science Frontier Research

Authors: Reda Hassan Amasha (PhD/Dr. count: 0)

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Publish Date: 2018 11, Fri

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