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Sperm sexing represents the most promising approach for production of genderspecific livestock under commercial settings. Flow cytometry is currently the method of choice for sexing sperm cells, but remains costly, has low throughput and lowers fertility in vivo. Thus, the study was aimed to test Percoll density centrifugation for sexing sperm cells in small ruminants. Semen from both rams (n = 4) and bucks (n = 4) was collected by electro-ejaculation, cryopreserved and further used for Percoll density centrifugation (PDC). Sperm sexing was confirmed by conventional PCR by determining the ratio of X and Y-bearing sperm cells. The semen before and after PDC in goats did not alter proportion of X-bearing (30%A vs 28%A; 34%A vs 41%A; 55%A vs 56%A; 42%A vs 40%A) or Y-bearing (70%A vs 72%A; 66%A vs 59%A; 45%A vs 44%A; 58%A vs 60%A).
Jose Carlos Ferreira-Silva. 2018. "Use of Percoll Density Centrifugation for Sperm sexing in Small Ruminants". Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 17 (GJSFR Volume 17 Issue D6).
Crossref Journal DOI 10.17406/GJSFR
Print ISSN 0975-5896
e-ISSN 2249-4626
v1.2
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Total Score: 107
Country: Brazil
Subject: Global Journal of Science Frontier Research - D: Agriculture & Veterinary
Authors: Jose Carlos Ferreira-Silva, Marcelo Tigre Moura, Sarah Romine Lima Basto, Luis Rennan Sampaio Oliveira, Eduardo Luiz Cavalcanti Caldas, Manoel Lopes Silva Filho , Marcos Antonio Lemos Oliveira (PhD/Dr. count: 0)
View Count (all-time): 373
Total Views (Real + Logic): 871
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Publish Date: 2017 10, Mon
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