Use of Percoll Density Centrifugation for Sperm sexing  in Small Ruminants

Use of Percoll Density Centrifugation for Sperm sexing in Small Ruminants

Jose Carlos Ferreira-Silva

Contact

Marcelo Tigre Moura

Contact

Sarah Romine Lima Basto

Contact

Luis Rennan Sampaio Oliveira

Contact

Eduardo Luiz Cavalcanti Caldas

Contact

Manoel Lopes Silva Filho

Contact

Marcos Antonio Lemos Oliveira

Contact

Universidade Federal Rural da Amazonia, Parauapebas-PA, Brazil.

Use of Percoll Density Centrifugation for Sperm sexing in Small Ruminants

Article Fingerprint

ReserarchID

3POY5

Use of Percoll Density Centrifugation for Sperm sexing in Small Ruminants Banner

AI TAKEAWAY

Connecting with the Eternal Ground

Abstract

Sperm sexing represents the most promising approach for production of genderspecific livestock under commercial settings. Flow cytometry is currently the method of choice for sexing sperm cells, but remains costly, has low throughput and lowers fertility in vivo. Thus, the study was aimed to test Percoll density centrifugation for sexing sperm cells in small ruminants. Semen from both rams (n = 4) and bucks (n = 4) was collected by electro-ejaculation, cryopreserved and further used for Percoll density centrifugation (PDC). Sperm sexing was confirmed by conventional PCR by determining the ratio of X and Y-bearing sperm cells. The semen before and after PDC in goats did not alter proportion of X-bearing (30%A vs 28%A; 34%A vs 41%A; 55%A vs 56%A; 42%A vs 40%A) or Y-bearing (70%A vs 72%A; 66%A vs 59%A; 45%A vs 44%A; 58%A vs 60%A).

References

23 Cites in Article

Reference Format

I Arruda,F Bezerra,J Silva,P Deus,C Aguiar-Filho,M Moura,P Lima,M Oliveira,D Rondina (2014). Effect of estrous type of teaser female on caprine sexual behavior and semen parameters.
J Chandler,M Wilson,A Canal,H Steinholt-Chenevert (1999). Bovine spermatozoal head size variation and evaluation of a separation technique based on this size.
Adauto Chiamenti,Cristiano Filho,Marcelo Moura,Fabíola Paula-Lopes,Jairo Neves,Cícero Neto,Paulo Gonçalves,Paulo Lima,Marcos Oliveira (2012). Use of retinyl acetate, retinoic acid and insulin-like growth factor-I (IGF-I) to enhance goat embryo production.
J Ferreira‐silva,Srl Basto,F Tenório Filho,M Moura,M Silva Filho,Mal Oliveira (2017). Reproductive performance of postpartum ewes treated with insulin or progesterone hormones in association with ram effect.
J Ferreira-Silva,T Burnett,P Souto,L Pereira,M Araujo,M Moura,M Oliveira (2017). Progesterone (P4), luteinizing hormone (LH) levels and ovarian activity in postpartum Santa Inês ewes subject to a male effect.
J Ferreira-Silva,M Moura,T Silva,L Oliveira,A Chiamenti,V Freitas,M Oliveira (2017). Full-term potential of goat in vitro produced embryos after different cryopreservation methods.
J Ferreira-Silva,M Chaves,F Tenório Filho,M Moura,L Neto,E Caldas,M Oliveira (2016). Reproductive efficiency of non-cycling postpartum ewes submitted to the male effect under tropical semi humid conditions.
W Hohenboken (1999). Applications of sexed semen in cattle production.
Rihachi Iizuka,Satoru Kaneko,Rui Aoki,Toshifumi Kobayashi (1987). Sexing of human sperm by discontinuous Percoll density gradient and its clinical application.
S Iwasaki,Y Shioya,H Masuda,A Hanada,T Nakahara (1988). Sex ratio of early embryos fertilized in vitro with spermatozoa separated by percoll.
L Johnson (1994). Isolation of X-and Y-bearing sperm for sex preselection.
Nilani Kanesharatnam,Thampoe Eswaramohan,Kandiah Balasubramaniam (2012). Fractionation of X and Y Chromosome-Bearing Bovine Spermatozoa through Sugar Gradients for Sex Predetermination in Dairy Cattle.
A Malik,A Haron,R Yusoff,M Bukar,A Kasim,M Sabri (2011). Verification of X-and Yspermatozoa separation by nested polymerase chain reaction (PCR), motility and membrane integrity in bovine.
L Morris,A Randall,W King,W Johnson,B Buckrell (2003). The contribution of the male to ovine embryogenesis in an in vitro embryo production system.
L Oliveira,M Moura,J Ferreira-Silva,P Nascimento,J Almeida-Irmão,L Neto,. Oliveira,M (2016). Nulliparous Anglo-Nubian goats submitted to male effect under contrasting climatic conditions.
M Palomo,D Izquierdo,T Mogas,M Paramio (1999). Effect of semen preparation on IVF of prepubertal goat oocytes.
A Phua,R Abdullah,Z Mohamed (2003). A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes.
M Resende,M Bezerra,F Perecin,A Almeida,A Lucio,V Lima (2009). Separation of X bearing bovine sperm by centrifugation in continuous percoll and optiprep density gradient: Effect in sperm viability and in vitro embryo production.
G Seidel (2003). Sexing mammalian spermintertwining of commerce, technology, and biology.
G Seidel (2007). Overview of sexing sperm.
B Solléro,D Faria,S Paiva,S Guimarães,P Lopes,D Paixão (2004). Método rápido de extração de DNA utilizando CTAB em tecidos musculares de suínos.
C Taylor,A Moore,R Thiessen,C Bailey (1985). Efficiency of food utilization in traditional and sex-controlled systems of beef production.
D Windsor,G Evans,I White (1993). Sex predetermination by separation of X and Y chromosome-bearing sperm: a review.

Download References

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Jose Carlos Ferreira-Silva. 2018. \u201cUse of Percoll Density Centrifugation for Sperm sexing in Small Ruminants\u201d. Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 17 (GJSFR Volume 17 Issue D6).

More Citation Formats

Select Citation Style:

Download Citation

GJSFR Volume 17 Issue D6

Explore Journals Explore Volume Read This Issue

Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

Not Found

Classification

GJSFR-D Classification FOR Code: QW 70

Submission ReceivedAugust 20, 2017
Peer Review Double Blind
Handling Editor
Accepted September 18, 2017
Published October 2, 2017

Version of record

v1.2

Issue date

January 20, 2018

Language

Experiance in AR

Explore published articles in an immersive Augmented Reality environment. Our platform converts research papers into interactive 3D books, allowing readers to view and interact with content using AR and VR compatible devices.

View in VR

Read in 3D

Your published article is automatically converted into a realistic 3D book. Flip through pages and read research papers in a more engaging and interactive format.

View in 3D

Article Matrices

Total Score: 107

Country: Brazil

Subject: Global Journal of Science Frontier Research - D: Agriculture & Veterinary

Authors: Jose Carlos Ferreira-Silva, Marcelo Tigre Moura, Sarah Romine Lima Basto, Luis Rennan Sampaio Oliveira, Eduardo Luiz Cavalcanti Caldas, Manoel Lopes Silva Filho , Marcos Antonio Lemos Oliveira (PhD/Dr. count: 0)

View Count (all-time): 181

Total Views (Real + Logic): 3325

Total Downloads (simulated): 1757

Publish Date: 2018 01, Sat

Monthly Totals (Real + Logic):

Month 1: 54 views
Month 2: 51 views
Month 3: 63 views
Month 4: 33 views
Month 5: 39 views
Month 6: 39 views
Month 7: 38 views
Month 8: 27 views
Month 9: 52 views
Month 10: 34 views
Month 11: 50 views
Month 12: 32 views
Month 13: 35 views
Month 14: 35 views
Month 15: 45 views
Month 16: 29 views
Month 17: 15 views
Month 18: 36 views
Month 19: 25 views
Month 20: 29 views
Month 21: 26 views
Month 22: 16 views
Month 23: 34 views
Month 24: 17 views
Month 25: 31 views
Month 26: 26 views
Month 27: 27 views
Month 28: 25 views
Month 29: 21 views
Month 30: 31 views
Month 31: 29 views
Month 32: 34 views
Month 33: 31 views
Month 34: 39 views
Month 35: 38 views
Month 36: 48 views
Month 37: 39 views
Month 38: 33 views
Month 39: 24 views
Month 40: 14 views
Month 41: 24 views
Month 42: 27 views
Month 43: 34 views
Month 44: 26 views
Month 45: 28 views
Month 46: 46 views
Month 47: 17 views
Month 48: 26 views
Month 49: 40 views
Month 50: 30 views
Month 51: 31 views
Month 52: 29 views
Month 53: 38 views
Month 54: 17 views
Month 55: 38 views
Month 56: 17 views
Month 57: 26 views
Month 58: 20 views
Month 59: 29 views
Month 60: 23 views
Month 61: 25 views
Month 62: 25 views
Month 63: 27 views
Month 64: 25 views
Month 65: 24 views
Month 66: 47 views
Month 67: 42 views
Month 68: 34 views
Month 69: 33 views
Month 70: 42 views
Month 71: 24 views
Month 72: 31 views
Month 73: 28 views
Month 74: 38 views
Month 75: 21 views
Month 76: 14 views
Month 77: 31 views
Month 78: 33 views
Month 79: 30 views
Month 80: 41 views
Month 81: 28 views
Month 82: 37 views
Month 83: 19 views
Month 84: 39 views
Month 85: 21 views
Month 86: 30 views
Month 87: 44 views
Month 88: 20 views
Month 89: 43 views
Month 90: 41 views
Month 91: 50 views
Month 92: 28 views
Month 93: 14 views
Month 94: 41 views
Month 95: 24 views
Month 96: 40 views
Month 97: 32 views
Month 98: 51 views
Month 99: 39 views
Month 100: 24 views

Total Views: 3325

Total Downloads: 1757

2026 Trends

Our website is actively being updated, and changes may occur frequently. Please clear your browser cache if needed. For feedback or error reporting, please email [email protected]