Use of Percoll Density Centrifugation for Sperm sexing in Small Ruminants

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Use of Percoll Density Centrifugation for Sperm sexing  in Small Ruminants

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Abstract

Sperm sexing represents the most promising approach for production of genderspecific livestock under commercial settings. Flow cytometry is currently the method of choice for sexing sperm cells, but remains costly, has low throughput and lowers fertility in vivo. Thus, the study was aimed to test Percoll density centrifugation for sexing sperm cells in small ruminants. Semen from both rams (n = 4) and bucks (n = 4) was collected by electro-ejaculation, cryopreserved and further used for Percoll density centrifugation (PDC). Sperm sexing was confirmed by conventional PCR by determining the ratio of X and Y-bearing sperm cells. The semen before and after PDC in goats did not alter proportion of X-bearing (30%A vs 28%A; 34%A vs 41%A; 55%A vs 56%A; 42%A vs 40%A) or Y-bearing (70%A vs 72%A; 66%A vs 59%A; 45%A vs 44%A; 58%A vs 60%A).

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Jose Carlos Ferreira-Silva. 2018. "Use of Percoll Density Centrifugation for Sperm sexing in Small Ruminants". Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 17 (GJSFR Volume 17 Issue D6).

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords
Classification
GJSFR-D Classification FOR Code: QW 70
Version of record

v1.2

Issue date
January 20, 2018

Language
English
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Use of Percoll Density Centrifugation for Sperm sexing in Small Ruminants

Jose Ferreira-Silva
Jose Ferreira-Silva Universidade Federal Rural da Amazonia, Parauapebas-PA, Brazil.