Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant

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olabisi_olufunmilayo_ogunrinola
olabisi_olufunmilayo_ogunrinola
2
Olabisi Olufunmilayo Ogunrinola
Olabisi Olufunmilayo Ogunrinola
3
Olusegun Omolade Fajana
Olusegun Omolade Fajana
4
Olaseni Pascal Oresanya
Olaseni Pascal Oresanya
1 Lagos State University

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Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant Banner
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The plant latex consist of biologically active compounds useful for diverse health benefits and could be a potential source of unique proteases. Proteases play key roles in the regulation of biological processes in plants and currently have become a vital part of the food and feed industry. In order to understand the underlying mechanisms of action of unique plant latex, the present study isolate and characterized a protease enzyme from the crude latex of plantain plant. Crude latex collected from different parts (leaves, stem, trunk and branches) of the plantain plant was centrifuge to separate the supernatant from any particles. The supernatant was used as the crude latex extract for the research. A protease enzyme was isolated from the crude latex extract and the effect of pH, temperature, incubation time, kinetic study of the protease activity as well as protein were determined spectrophoto-metrically. The enzyme protease was found to have a 9.84 mg/ml of protein with a specific activity of 3.06 unit/mg protein respectively.

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

olabisi_olufunmilayo_ogunrinola. 2021. \u201cKinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 21 (GJSFR Volume 21 Issue C1): .

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GJSFR Volume 21 Issue C1
Pg. 25- 30
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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification: FOR Code: 060199
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v1.2

Issue date

March 8, 2021

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English

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The plant latex consist of biologically active compounds useful for diverse health benefits and could be a potential source of unique proteases. Proteases play key roles in the regulation of biological processes in plants and currently have become a vital part of the food and feed industry. In order to understand the underlying mechanisms of action of unique plant latex, the present study isolate and characterized a protease enzyme from the crude latex of plantain plant. Crude latex collected from different parts (leaves, stem, trunk and branches) of the plantain plant was centrifuge to separate the supernatant from any particles. The supernatant was used as the crude latex extract for the research. A protease enzyme was isolated from the crude latex extract and the effect of pH, temperature, incubation time, kinetic study of the protease activity as well as protein were determined spectrophoto-metrically. The enzyme protease was found to have a 9.84 mg/ml of protein with a specific activity of 3.06 unit/mg protein respectively.

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Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant

Olabisi Olufunmilayo Ogunrinola
Olabisi Olufunmilayo Ogunrinola
Olusegun Omolade Fajana
Olusegun Omolade Fajana
Olaseni Pascal Oresanya
Olaseni Pascal Oresanya

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