Antioxidant Activity and Total Phenolic Content of Eucheuma cottonii and Sargassum sp. From South Sulawesi Indonesia

α
Syaharuddin
Syaharuddin
σ
Natalia Rombe
Natalia Rombe
ρ
Yosefa M
Yosefa M
Ѡ
Marianti Manggau
Marianti Manggau
¥
Rosany Tayeb
Rosany Tayeb
§
Sudir Sumarheni
Sudir Sumarheni
α Hasanuddin University

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Antioxidant Activity and Total Phenolic Content of Eucheuma cottonii and Sargassum sp. From South Sulawesi Indonesia

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Abstract

Phenolic compounds have been extracted from red algae (Eucheuma cottonii) and brown algae (Sargassum sp.) and the antioxidant activity assay using DPPH method has been done. The extractions were carried out on dried and fresh samples by digestion method using ethanol: water (50:50) as solvents and followed by liquid-liquid extraction using hexane and ethyl acetate as solvents. The extraction results were qualitatively analyzed using Folin-ciocalteu and TLC methods, while quantitative analysis was performed by determination of total polyphenol levels and antioxidant activity using UV-Vis spectrophotometer. Identification with Folinciocalteu reagent showed the presence of polyphenol compounds in the ethanol fraction and ethyl acetate fraction. The TLC results showed a spot with a relatively equal Rf value between ethyl acetate fraction and gallic acid standard in both samples. The highest polyphenol content was obtained from the ethyl acetate fraction of fresh samples, 0.59% for red algae and 0.34% for brown algae while the ethanol fraction of dried samples had the lowest polyphenol content of 0.08% for red algae and 0.01% for brown algae.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Syaharuddin. 2020. \u201cAntioxidant Activity and Total Phenolic Content of Eucheuma cottonii and Sargassum sp. From South Sulawesi Indonesia\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 20 (GJSFR Volume 20 Issue C1): .

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Issue Cover
GJSFR Volume 20 Issue C1
Pg. 21- 26
Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification: FOR Code: 069999
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v1.2

Issue date

February 11, 2020

Language
en
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Phenolic compounds have been extracted from red algae (Eucheuma cottonii) and brown algae (Sargassum sp.) and the antioxidant activity assay using DPPH method has been done. The extractions were carried out on dried and fresh samples by digestion method using ethanol: water (50:50) as solvents and followed by liquid-liquid extraction using hexane and ethyl acetate as solvents. The extraction results were qualitatively analyzed using Folin-ciocalteu and TLC methods, while quantitative analysis was performed by determination of total polyphenol levels and antioxidant activity using UV-Vis spectrophotometer. Identification with Folinciocalteu reagent showed the presence of polyphenol compounds in the ethanol fraction and ethyl acetate fraction. The TLC results showed a spot with a relatively equal Rf value between ethyl acetate fraction and gallic acid standard in both samples. The highest polyphenol content was obtained from the ethyl acetate fraction of fresh samples, 0.59% for red algae and 0.34% for brown algae while the ethanol fraction of dried samples had the lowest polyphenol content of 0.08% for red algae and 0.01% for brown algae.

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Antioxidant Activity and Total Phenolic Content of Eucheuma cottonii and Sargassum sp. From South Sulawesi Indonesia

Syaharuddin
Syaharuddin Hasanuddin University
Natalia Rombe
Natalia Rombe
Yosefa M
Yosefa M
Marianti Manggau
Marianti Manggau
Rosany Tayeb
Rosany Tayeb
Sudir Sumarheni
Sudir Sumarheni

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