Occurrence of Escherichia Coli and Staphylococcus Aureus in Sliced Pineapple and Water Melon Fruits Sold in Akpan Andem Market, Uyo, Akwa Ibom State, Nigeria

Occurrence of Escherichia Coli and Staphylococcus Aureus in Sliced Pineapple and Water Melon Fruits Sold in Akpan Andem Market, Uyo, Akwa Ibom State, Nigeria

Prof. Abomaye-Nimenibo Williams Aminadokiari Samuel Ph.D., M.Sc., B.Sc. Economics, MBA Management and UD, Personnel Management and Industrial Relations, Director of Postgraduate Studies, School of Postgraduate Studies

Contact

Dr. Ikon

Contact

Grace M.

Contact

Jackson

Contact

Roseline Idorenyin

Contact

Obong University

Occurrence of Escherichia Coli and Staphylococcus Aureus in Sliced Pineapple and Water Melon Fruits Sold in Akpan Andem Market, Uyo, Akwa Ibom State, Nigeria

Article Fingerprint

ReserarchID

572SP

AI TAKEAWAY

Connecting with the Eternal Ground

Abstract

The aim of this study was to carry microbiological assessment of the prevalence of Staphylococcus aureus and Escherichia coli associated sliced ready-to-eat water melon and pineapple fruits. A total of 10 sliced pineapple and water melon fruits were purchased from different vendors at Akpan Andem Market, Uyo. The fruit samples were aseptically transported to the laboratory and processed using standard techniques. Enumeration of total heterotrophic bacteria and fungi, and isolation, characterization and identification of Staphylococcus aureus and Escherichia coli were all done using standard microbiological techniques. Antibiotic sensitivity test was carried out using the Kirby Bauer disk diffusion test. The results of the aerobic bacterial and fungal counts indicated that the highest bacterial count was 1.85 x 10 4 for water melon and 1.80 x 10 4 cfu/g for pineapple while those of the fungi were 1.3 and 1.4 x 10 3 cfu/g, respectively. For E. coli, the least and highest zones of inhibitions were 6.00±1.05 and 23.37±1.20 mm for isolates WS5 and PA3, respectively against CEP and CPX which were Ciprofloxacin and Ceporex. A total of 4 out of 10 isolates showed multi-drug resistance (resistance to at least 2 antibiotics) and these were isolates PA4, PA1, WS4, and WS5.

References

45 Cites in Article

Reference Format

A Abubakari,I Amoah,G Essiaw-Quayson,J Larbi,R Seidu,R Abaidoo (2015). Presence of pathogenic E. coli in ready-to-eat salad food from vendors in the Kumasi metropolis, Ghana.
B Agbo,N Udoekong,R Ozumba (2016). The Incidence of Staphylococcus aureus in Fresh Meat sold at Evening Markets within Yenagoa Metropolis, Bayelsa State, Nigeria.
N Barro,A Bello,S Aly,C Ouattara,A Ilboudo,Jules,A Traoré (2006). Hygienic status an ssessment of dishwashing waters, utensils, hands and pieces of money from treet food processing sites in Ouagadougou (Burkina Faso).
D Bartholomew,R Paul,K Rorbach (2003). The pineapple 'Botany, Production and Uses.
O Baruwa (2013). Profitability and constraints of pineapple production in Osun State, Nigeria.
G Brooks,K Carroll,S Butel,S Morses,T Meitzner (2010). Morphology and identification of Staphylococcus aureus in: Jawetz, Melnickth and Adelberg's Medical Microbiology.
(2006). Analysis and presentation of cumulative antimicrobial susceptibility test data.
P Debnath,P Dey,A Chanda,T Bhakta (2012). A Survey on Pineapple and its medicinal value.
M Dia (2012). Genotype × Environment Interaction and Stability Analysis of Performance, and Mega-Environment Identification of Fruit Yield and Yield Components in Watermelon [Citrullus lanatus (Thumb.) Matsum & Nakai] Tested in Multiple US Locations.
Mahendra Dia,Todd Wehner,Richard Hassell,Daniel Price,George Boyhan,Stephen Olson,Stephen King,Angela Davis,Gregory Tolla,Jerome Bernier,Benito Juarez (2012). Value of Locations for Representing Mega‐Environments and for Discriminating Yield of Watermelon in the U.S..
M Dia,T Wehner,R Hassell,D Price,G Boyhan,S Olson,S King,A Davis,G Tolla,J Bernier,B Juarez,N Sari,I Solmaz,V Aras (2012). Stability of fruit yield in watermelon genotypes tested in multiple US environments.
M Dia,T Wehner,R Hassell,D Price,G Boyhan,S Olson,S King,A Davis,G Tolla (2016). Genotype × environment interaction and stability analysis for watermelon fruit yield in the U.
Mahendra Dia,Todd Wehner,Richard Hassell,Daniel Price,George Boyhan,Stephen Olson,Stephen King,Angela Davis,Gregory Tolla,Jerome Bernier,Benito Juarez (2016). Value of Locations for Representing Mega‐Environments and for Discriminating Yield of Watermelon in the U.S..
Mahendra Dia,Todd Wehner,Penelope Perkins-Veazie,Richard Hassell,Daniel Price,George Boyhan,Stephen Olson,Stephen King,Angela Davis,Gregory Tolla,Jerome Bernier,Benito Juarez (2016). Stability of fruit quality traits in diverse watermelon cultivars tested in multiple environments.
L Do Carmo,C Cummings,V Linardi,D Souza,J De Souza,M De Sena,D Dos Santos,J Shupp,R Peres Pereira,M Jett (2004). A case study of a massive staphylococcal food poisoning incident.
U Edet,S Antai,A Brooks,A Asitok (2017). An Overview of Cultural, Molecular and Metagenomics Techniques in Description of Microbial Diversity.
Alison Edwards,Eugene Wiley,Ellen Brown,Beverly Clevidence,Bryan Vinyard,Julie Collins,Penelope Perkins-Veazie,Robert Baker (2003). Consumption of Watermelon Juice Increases Plasma Concentrations of Lycopene and β-Carotene in Humans.
G Ekici,E Dumen (2004). Escherichia coli and Food Safety.
(2005). OECD-FAO Agricultural Outlook 2005.
A Hennekinne,M De Busuyer,S Dragacci (2012). Staphylococcus aureus and its food poisoning toxins: characterization and outbreak investigation.
C Holzel,. Tetens,K Schwaiger (2018). Unraveling the Role of Vegetables in Spreading Antimicrobial-Resistant Bacteria: A Need f or Quantitative Risk Assessment.
Jisoo Hong,Yangkyun Kim,Jonguk Kim,Sunggi Heu,Se‐ri Kim,Kwang‐pyo Kim,Eunjung Roh (2015). Genetic Diversity and Antibiotic Resistance Patterns of <i>Staphylococcus Aureus</i> Isolated from Leaf Vegetables in Korea.
A Kader,F Hossain,M Islam,G Kabir,S Sarker,N Absar (2010). A Comparative analysis on the nutritional contents of two varieties of pineapple of Chittagong region.
A Kalia,R Gupta (2006). Handbook of fruit and fruit processing, 1 st Edition.
R Kumar,M Dia,T Wehner (2013). Implications of mating behavior in watermelon breeding.
G Kwaku,S Samson,P Mills-Robertson,F (2016). Resistance of Bacteria Isolates from Cabbage (Brassica oleracea), Carrots (Daucus carota) and Lettuce (Lactuca sativa) in the Kumasi Metropolis of Ghana.
J Le,J Chuan,H Andy,W Colin (2005). Do dietary lycopene and other carotenoids protect against prostate cancer.
Srinath D. (2008). Microbiological Quality Analysis of Street Vended Fruit Juices in Hyderabad City, India.
P Mensah,K Owusu-Darko,D Yeboah-Manu,A Ablordey,F Nkrumah,H Kamiya (1999). The role of street food vendors in transmission of enteric pathogens.
A Nielsen (2006). DETERMINATION OF SOME HEAVY METALS IN SOME READY-TO-EAT MEALS IN ASSIUT CITY.
O Okechukwu,C Orinya,E Okonkwo,C Uzoh,U Ekuma,G Onuh,E (2016). The Microbial Contamination of Ready-To-Eat Vended Fruits in Abakpa Main Market, Abakaliki, Ebonyi State, Nigeria.
T Sharomi (2013). Microbiological Quality of Street-Vended Foods and Ready-To-Eat Vegetables in Some Nigeria Cities.
O Oseni,V Okoye (2013). Studies of phytochemical and antioxidant properties of the fruit of watermelon (Citrullus lanatus). (Thunb).
L Prescott,J Harley,D Klein (1999). An introduction to biological evolution Kenneth V.. Kardong . 2008.<i>An introduction to biological evolution.</i>Second Edition. The McGraw-Hill Companies, Inc.. New York, New York, U.S.A.. 352ISBN: 978-0-07-305077-5. Soft cover..
M Rahmatullah,I Mukti,A Haque,M Mollik,K Parvin,R Jahan,M Chowdhury,T Rahman (2009). An ethno-botanical survey and pharmacological evaluation of medicinal plants used by the Garo tribal community living in Netrakona district, Bangladesh.
S Rakesh,P Patil,S Mane (2010). Use of natural antioxidants to scavenge free radicals: A major cause of diseases.
T Reetu,M Tomar (2017). Watermelon: A Valuable Horticultural Crop with Nutritional Benefits.
A Rimando,P Perkins-Veazie (2005). Determination of citrulline in watermelon rind.
K Sabahelkhier,A Hussain,K Ishag (2010). Table 1: Protein information of pineapple (Ananas comosus) heat shock transcription factors (AcHSFs)..
Om Sharma,Tej Bhat (2009). DPPH antioxidant assay revisited.
D Tambekar,D Dhanorkar,S Gulhane,M Dudhane (2009). Prevalence, Profile and Antibiotic Susceptibility Pattern of Bacterial Isolates from Blood.
A Titamare,P Dabholkar,Godbole (2009). Bacteriological analysis of street vended fresh fruit and vegetable juices in Nagpur City, India.
Shih-Chun Yang,Chih-Hung Lin,Ibrahim Aljuffali,Jia-You Fang (2017). Current pathogenic Escherichia coli foodborne outbreak cases and therapy development.
T Wehner,N Shetty,G Elmstrom (2001). Breeding and seed production.
Who (2002). Food safety and food borne illness.

Download References

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Prof. Abomaye-Nimenibo Williams Aminadokiari Samuel. 2020. \u201cOccurrence of Escherichia Coli and Staphylococcus Aureus in Sliced Pineapple and Water Melon Fruits Sold in Akpan Andem Market, Uyo, Akwa Ibom State, Nigeria\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 20 (GJSFR Volume 20 Issue C2): .

More Citation Formats

Select Citation Style:

Download Citation

Download Article

GJSFR Volume 20 Issue C2
Pg. 1- 15

Explore Journals Explore Volume Read This Issue

Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

Not Found

Classification

GJSFR-C Classification: FOR Code: 069999

Submission ReceivedJanuary 6, 2020
Peer Review Double Blind
Handling Editor
Accepted February 4, 2020
Published February 16, 2020

Version of record

v1.2

Issue date

March 16, 2020

Language

Experiance in AR

Explore published articles in an immersive Augmented Reality environment. Our platform converts research papers into interactive 3D books, allowing readers to view and interact with content using AR and VR compatible devices.

View in VR

Read in 3D

Your published article is automatically converted into a realistic 3D book. Flip through pages and read research papers in a more engaging and interactive format.

View in 3D

Article Matrices

Total Views: 2649

Total Downloads: 1266

2026 Trends

Published Article

Our website is actively being updated, and changes may occur frequently. Please clear your browser cache if needed. For feedback or error reporting, please email [email protected]