Transcriptomic Profiling of Pseudomonas Putida (NBAII RPF9) Exposed to Heat and Salt Shock

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Ashwitha K
Ashwitha K
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Rangeshwaran
Rangeshwaran

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Transcriptomic Profiling of Pseudomonas Putida (NBAII RPF9) Exposed to Heat and Salt Shock

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Abstract

Pseudomonas spp. is one of the widely studied bacterium for its versatility to be used for agricultural purposes as bioagent, biostimulant, biofertilizers or biopesticides. However, the dynamics of its efficacy varied from lab to field. The advanced biocontrol technologies have enabled better understanding of adaptability of selected Pseudomonas for field applications. In our study, we subjected P.putida (NBAII RPF9) strain to heat shock and salt shock followed by microarray analysis of the gene expression under both stresses when induced independently. Few genes were expressed under both conditions whereas some of them were newly reported stress tolerance genes. The quantification of selected stress tolerance genes revealed the level of fold change of these genes under stress and its role in conferring mitigation of heat and salt shock to P.putida (NBAII RPF 9). These techniques can be considered as major criterion in selection of a potential bacterial strain for agricultural applications.

References

34 Cites in Article
  1. Sk. Ali,V Sandhya,Minakshi Grover,N Kishore,L Rao,B Venkateswarlu (2009). Pseudomonas sp. strain AKM-P6 enhances tolerance of sorghum seedlings to elevated temperatures.
  2. Inés Arana,Alicia Muela,Maite Orruño,Carolina Seco,Idoia Garaizabal,Isabel Barcina (2010). Effect of temperature and starvation upon survival strategies of Pseudomonas fluorescens CHA0: comparison with Escherichia coli.
  3. K Ashwitha,R Rangeshwaran,N Vajid,G Sivakumar,S Jalali,K Rajalaxmi,K Manjunatha (2013). Characterization of abiotic stress tolerant Pseudomonas spp. occurring in Indian soils.
  4. Karkera Ashwitha,Rajagopal Rangeshwaran,Gopalsamy Sivakumar (2018). "Molecular mechanisms adopted by abiotic stress tolerant Pseudomonas fluorescens (NBAII-PFDWD) in response to in vitro osmotic stress".
  5. Deepak Balasubramanian,Kalai Mathee (2009). Comparative transcriptome analyses of Pseudomonas aeruginosa.
  6. H Ballerstedt,R Volkers,A Mars,J Hallsworth,V Santos,J Pchalka,J Durren,G Eggink,K Timmis,J Bont,J Wery (2007). Genomotyping of Pseudomonas putida strains using P. putida KT2440-based highdensity DNA microarrays: implications for transcriptomics studies.
  7. G Gülez,A Dechesne,C Workman,B Smets (2012). Transcriptome dynamics of Pseudomonas putida KT2440 under water stress.
  8. Thusitha Gunasekera,Laszlo Csonka,Oleg Paliy (2008). Genome-Wide Transcriptional Responses of<i>Escherichia coli</i>K-12 to Continuous Osmotic and Heat Stresses.
  9. Rainer Kappes,Bettina Kempf,Susanne Kneip,Jens Boch,Jutta Gade,Jana Meier-Wagner,Erhard Bremer (1999). Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis.
  10. Kenneth Livak,Thomas Schmittgen (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method.
  11. Farajollah Maleki,A Khosravi,A Nasser,H Taghinejad,M Azizian (2016). Bacterial Heat Shock Protein Activity.
  12. Mary Mcmillan,Lily Pereg (2014). Evaluation of Reference Genes for Gene Expression Analysis Using Quantitative RT-PCR in Azospirillum brasilense.
  13. Mary Mcmillan,Lily Pereg (2014). Evaluation of Reference Genes for Gene Expression Analysis Using Quantitative RT-PCR in Azospirillum brasilense.
  14. Shashwat Nagar,Bhavye Aggarwal,Shikha Joon,Rakesh Bhatnagar,Sonika Bhatnagar (2016). A Network Biology Approach to Decipher Stress Response in Bacteria Using <i>Escherichia coli</i> As a Model.
  15. R Noor,T Tabassum,T Tabassum,N Tabassum,S Maniha (2019). Growth and Survival of Pseudomonas sp. under cold shock.
  16. Gabriele Berg (2015). Beyond borders: investigating microbiome interactivity and diversity for advanced biocontrol technologies.
  17. J Boch,G Nau-Wagner,S Kneip,E Bremer (1997). Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine.
  18. Gregory Challis,Jacques Ravel,Craig Townsend (2000). Predictive, structure-based model of amino acid recognition by nonribosomal peptide synthetase adenylation domains.
  19. Kok-Gan Chan,Kumutha Priya,Chien-Yi Chang,Ahmad Abdul Rahman,Kok Tee,Wai-Fong Yin (2016). Transcriptome analysis of<i>Pseudomonas aeruginosa</i>PAO1 grown at both body and elevated temperatures.
  20. N Choudhury,I T Nur,M Munna,R Noor,G Novik,V Savich,E Kiseleva (2015). Comparative study of oxidative stress response in Pseudomonas aeruginosa {Subp01} 23.
  21. Stephan Pabinger,Stefan Rödiger,Albert Kriegner,Klemens Vierlinger,Andreas Weinhäusel (2014). A survey of tools for the analysis of quantitative PCR (qPCR) data.
  22. K Peterson (2009). Evaluation of the expression of water stress responsive Pseudomonas syringae genes during plant infection and in the presence of low osmotic versus low matric potential in culture.
  23. R Rangeshwaran,K Ashwitha,G Sivakumar,S Jalali (2013). Analysis of Proteins Expressed by an Abiotic Stress Tolerant Pseudomonas putida (NBAII-RPF9) Isolate Under Saline and High Temperature Conditions.
  24. Mercedes Reina-Bueno,Montserrat Argandoña,Manuel Salvador,Javier Rodríguez-Moya,Fernando Iglesias-Guerra,Laszlo Csonka,Joaquín Nieto,Carmen Vargas (2012). Role of Trehalose in Salinity and Temperature Tolerance in the Model Halophilic Bacterium Chromohalobacter salexigens.
  25. Klaus Richter,Martin Haslbeck,Johannes Buchner (2010). The Heat Shock Response: Life on the Verge of Death.
  26. Erica Ricker,Haydar Aljaafari,Trigg Bader,Bryce Hundley,Eric Nuxoll (2018). Thermal shock susceptibility and regrowth of<i>Pseudomonas aeruginosa</i>biofilms.
  27. Surendra Singh,Chad Brocker,Vindhya Koppaka,Ying Chen,Brian Jackson,Akiko Matsumoto,David Thompson,Vasilis Vasiliou (2013). Aldehyde dehydrogenases in cellular responses to oxidative/electrophilicstress.
  28. F Spiegelhalter,E Bremer (1998). Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma Aand sigma B-dependent stress responsive promoters.
  29. A Swiecelo,I Zych-Wezyk (2013). Bacterial Stress Response as an Adaptation to Life in a Soil Environment.
  30. Roberto Velasco-García,Miguel Villalobos,Miguel Ramírez-Romero,Carlos Mújica-Jiménez,Gabriel Iturriaga,Rosario Muñoz-Clares (2006). Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa: cloning, over-expression in Escherichia coli, and regulation by choline and salt.
  31. Yong Wang,Sho Morimoto,Naoto Ogawa,Takeshi Fujii (2011). A survey of the cellular responses in Pseudomonas putida KT2440 growing in sterilized soil by microarray analysis.
  32. D Weller (1988). Biological control of soil borne plant pathogens in the rhizosphere with bacteria.
  33. Lynn Wood,Dennis Ohman (2015). Cell wall stress activates expression of a novel stress response facilitator (SrfA) under σ22 (AlgT/U) control in Pseudomonas aeruginosa.
  34. Liang Yin,Yanfen Xue,Yanhe Ma (2015). Global Microarray Analysis of Alkaliphilic Halotolerant Bacterium Bacillus sp. N16-5 Salt Stress Adaptation.

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Ashwitha K. 2020. \u201cTranscriptomic Profiling of Pseudomonas Putida (NBAII RPF9) Exposed to Heat and Salt Shock\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 20 (GJSFR Volume 20 Issue C5): .

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification: FOR Code: 279999
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v1.2

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September 28, 2020

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en
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Pseudomonas spp. is one of the widely studied bacterium for its versatility to be used for agricultural purposes as bioagent, biostimulant, biofertilizers or biopesticides. However, the dynamics of its efficacy varied from lab to field. The advanced biocontrol technologies have enabled better understanding of adaptability of selected Pseudomonas for field applications. In our study, we subjected P.putida (NBAII RPF9) strain to heat shock and salt shock followed by microarray analysis of the gene expression under both stresses when induced independently. Few genes were expressed under both conditions whereas some of them were newly reported stress tolerance genes. The quantification of selected stress tolerance genes revealed the level of fold change of these genes under stress and its role in conferring mitigation of heat and salt shock to P.putida (NBAII RPF 9). These techniques can be considered as major criterion in selection of a potential bacterial strain for agricultural applications.

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Transcriptomic Profiling of Pseudomonas Putida (NBAII RPF9) Exposed to Heat and Salt Shock

Ashwitha K
Ashwitha K
Rangeshwaran
Rangeshwaran
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