Use of NMR to Determine Compatable Solutes in Halophilic Bacteria Isolated from Highly Saline Areas

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Reda Hassan Amasha
Reda Hassan Amasha
α King Abdulaziz University King Abdulaziz University

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Use of NMR to Determine Compatable Solutes in Halophilic Bacteria Isolated from Highly Saline Areas

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Abstract

Ten halophilic bacteria (two Gram-negative) belonging to the Halomonadaceae and (eight Gram-positive), belonging to the Bacillaceae, were isolated fromtheRed Sea, Arabian Gulf and Dead Sea using a high salinity medium, followed by identification using 16S rRNA.Four of the isolates were designated on the basis of their tolerance to highsalinity. The isolates respectivlyexhibited 97% homology to Halomonasaquamarina, 97% homology to Sediminibacillus sp., (Red Sea), 94% homology to Halobacillus sp., (Arabian Gulf) and 98% homology to Halobacillusdabanensis(Dead Sea). 1H-NMR spectroscopy was used to determine the osmolytes accumulated by H. aquamarina, Sediminibacillus sp.,Halobacillus sp. and H. dabanensis grown in a saline nutrient medium at varying concentrations of NaCl and a range of organic sources. In the case of H. aquamarina, betaine and ectoine concentrations increased at high salinities. In contrast, betaine was found when casein and peptone were used as nutrient sources, while ectoine was produced in the presence of peptone.

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Reda Hassan Amasha. 2019. \u201cUse of NMR to Determine Compatable Solutes in Halophilic Bacteria Isolated from Highly Saline Areas\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 19 (GJSFR Volume 19 Issue C1): .

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Issue Cover
GJSFR Volume 19 Issue C1
Pg. 13- 23
Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification: FOR Code: 060599
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v1.2

Issue date

March 7, 2019

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en
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Ten halophilic bacteria (two Gram-negative) belonging to the Halomonadaceae and (eight Gram-positive), belonging to the Bacillaceae, were isolated fromtheRed Sea, Arabian Gulf and Dead Sea using a high salinity medium, followed by identification using 16S rRNA.Four of the isolates were designated on the basis of their tolerance to highsalinity. The isolates respectivlyexhibited 97% homology to Halomonasaquamarina, 97% homology to Sediminibacillus sp., (Red Sea), 94% homology to Halobacillus sp., (Arabian Gulf) and 98% homology to Halobacillusdabanensis(Dead Sea). 1H-NMR spectroscopy was used to determine the osmolytes accumulated by H. aquamarina, Sediminibacillus sp.,Halobacillus sp. and H. dabanensis grown in a saline nutrient medium at varying concentrations of NaCl and a range of organic sources. In the case of H. aquamarina, betaine and ectoine concentrations increased at high salinities. In contrast, betaine was found when casein and peptone were used as nutrient sources, while ectoine was produced in the presence of peptone.

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Use of NMR to Determine Compatable Solutes in Halophilic Bacteria Isolated from Highly Saline Areas

Reda Hassan Amasha
Reda Hassan Amasha King Abdulaziz University

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