Standardization of Extraction Techniques of Picroside-I and Picroside-II from aoKutkia (Picrorhiza Kurroa Royle Ex Benth.)

1
Parbat Raj Thani
Parbat Raj Thani
2
Yash Pal Sharma
Yash Pal Sharma
3
Prashid Kandel
Prashid Kandel
4
Kriti Nepal
Kriti Nepal
1 Dr. Y. S. Parmar University of Forestry and Horticulture

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Kutki (Picrorhiza kurroa Royle ex Benth.) is an important medicinal plant used in various herbal drug formulations possessing hepatoprotective activity. The pharmacological importance of this species has been demonstrated due to the presence of iridoid glycosides, such as picroside-I and picroside-II in underground part. In the present study, fresh roots and rhizomes collected from 2600m to 3300m altitude of Himachal Pradesh (Rohtang area) were used and extract yield, phytochemical constituents, namely picroside-I and picroside-II, of water and methanol extracts prepared using four different extraction methods viz. soxhlet, extraction by refluxing, microwave assisted extraction and sonication assisted extraction were compared for the standardization of extraction methods of picroside-I and picroside-II. Sonication assisted extraction for 36 minutes with methanol as solvent yielded 44.269 per cent extract with 6.825 per cent picroside-I and 5.291 per cent picroside-II content, which was better in comparison to other methods regarding time consumption and yield.

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The authors declare no conflict of interest.

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Parbat Raj Thani. 2018. \u201cStandardization of Extraction Techniques of Picroside-I and Picroside-II from aoKutkia (Picrorhiza Kurroa Royle Ex Benth.)\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 18 (GJSFR Volume 18 Issue C1): .

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GJSFR Volume 18 Issue C1
Pg. 51- 56
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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification: FOR Code: 070599
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v1.2

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April 30, 2018

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English

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Kutki (Picrorhiza kurroa Royle ex Benth.) is an important medicinal plant used in various herbal drug formulations possessing hepatoprotective activity. The pharmacological importance of this species has been demonstrated due to the presence of iridoid glycosides, such as picroside-I and picroside-II in underground part. In the present study, fresh roots and rhizomes collected from 2600m to 3300m altitude of Himachal Pradesh (Rohtang area) were used and extract yield, phytochemical constituents, namely picroside-I and picroside-II, of water and methanol extracts prepared using four different extraction methods viz. soxhlet, extraction by refluxing, microwave assisted extraction and sonication assisted extraction were compared for the standardization of extraction methods of picroside-I and picroside-II. Sonication assisted extraction for 36 minutes with methanol as solvent yielded 44.269 per cent extract with 6.825 per cent picroside-I and 5.291 per cent picroside-II content, which was better in comparison to other methods regarding time consumption and yield.

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Standardization of Extraction Techniques of Picroside-I and Picroside-II from aoKutkia (Picrorhiza Kurroa Royle Ex Benth.)

Parbat Raj Thani
Parbat Raj Thani Dr. Y. S. Parmar University of Forestry and Horticulture
Yash Pal Sharma
Yash Pal Sharma
Prashid Kandel
Prashid Kandel
Kriti Nepal
Kriti Nepal

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