Serotyping of Salmonella Enterica Isolated from Broiler Chicks in an Outbreak in Sudan
Aims: This study investigates the first emergence of S almonella outbreaks among 8.000 broiler chicks, of the ‘ Ross’ breed, in Sudan. Mortality was observed in 25% of the t otal chicks; therefore, samples were taken for culturing, i dentification and characterization of the causative agent. Study design: A total of 52 random samples, out of 2000 i nfected broiler chicks, were cultured and identified. The study was undertaken in the laboratories of the Veterinary Research Institute, Ministry of animal resources and fisheries, Khartoum as well as Department of Botany, Faculty of Science, University of Khartoum, Sudan during March 2014- December 2014. Results: All of the 52 isolates have shown colony characteristic t ypical to Salmonella spp. Result of the Vitek2 compact system s howed that isolate was typical Salmonella enterica. The p rimer pairs targeting invA and hilA genes successfully a mplified the extracted DNA giving the specific amplicons.
