Effect of Cryopreservation on Biological Markers of Sperm Function and their Correlation in Different Dog Breeds
In the present study, we compared motility, viability, plasma membrane integrity (PMI), acrosome integrity (AI), inner mitochondrial membrane potential (IMMP), ATP and LDHC in freshly extended (FE,) and frozen-thaw (FT) semen among the dogs and breeds. Freshly extended semen in Tris-citric acid-fructose-egg yolk plasma-glycerol extenderwas also subjected to in vitro capacitation and acrosome reaction. Pearson correlation coefficient among the sperm attributes of FE and FT semen has been evaluated.Sperm attributes in FE and FT semen vary significantly (P<0.05) among the dogs. There was a significant (P≤0.05) decline in motility, viability PMI, AI and high IMMP (HIMMP)during freezing-thawing process, in contrast, in ATP, LDHC and medium IMMP(MIMMP)decline was non-significant (P≥0.05). There was a loss of 21.7-36.7%, 20.9-36.6%, 20.3-53.6%, 18.9-43.9%, 16.1-36% in motility, viability, PMI, AI and HIMMP during freezing-thawing process. A loss in ATP and LDHC content was also observed during freezing-thawing process. Incubation of washed spermatozoa of 13 dogs in canine TALP medium for 6 hrs resulted in a decline in motility, viability, MIMMP and an increase in HIMMP and percentage of the altered acrosome.
