Compositional Analysis, Antioxidant and Antimicrobial Potential of the Seed of Annona cinerea Dunal Grown in Nigeria
Seed of Annona cinerea grown in Nigeria was investigated for its secondary metabolites and antioxidant potential using Gas Chromatography-Mass Spectrometry (GC-MS), 2,2ʹ- dyphenyl-1-picrylhydrazyl (DPPH) and 2,6-ditert-butyl-4-[(3,5-ditert-butyl-4-λ1- oxidanylphenyl)methylidene] cyclohexa-2,5-dien-1-one (Galvinoxyl), respectively. The antibacterial activity of the seed extract was evaluated on eleven (11) pathogenic bacteria using agar well diffusion method at different concentrations of the extract. Twenty-seven (27) therapeutically active secondary metabolites were identified in the seed extract using GC-MS and the principal constituents identified were 3-O-methyl-d-glucose (52.14%), β-sitosterol (11.79%), desulphosinigrin (6.16%) and α-tocopherol (5.84%). The extract also displayed high DPPH and galvinoxyl radical scavenging activity with IC50 values of 5.0 and 100 μgml-1. The zones of inhibition ranged from 10-30 mm against all tested bacteria. The antibacterial index (AI) ranged between 0.5-25. This study demonstrated that the seed of A. cinerea could be a potential source of natural antioxidants and antimicrobial agents.
