Use of Different Immunoresponse Assays for Evaluation of Live Attenuated Sheep Pox Vaccine in Comparison with Challenge Test

Use of Different Immunoresponse Assays for Evaluation of Live Attenuated Sheep Pox Vaccine in Comparison with Challenge Test

Sonia A Rizk

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Nermeen G. Shafik

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Ibrahim M.M

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Sonia A. Rizk

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Ali A.M

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Veterinary Serum and Vaccine Research Institute

Use of Different Immunoresponse Assays for Evaluation of Live Attenuated Sheep Pox Vaccine in Comparison with Challenge Test

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Abstract

Sheep pox (SP) is one of the priorities, high-impact animal diseases in many developing countries, where live attenuated vaccines are routinely used against sheep pox virus (SPV). Sheep pox virus is a member of the family Poxviridae, genus Capri poxvirus. In this study, live attenuated Sheep pox vaccines were evaluated for humoral and cellular immunity using virus neutralization index (NI), ELISA and lymphocyte proliferation assay (XTT) beside routinely titration of life attenuated virus content of vaccine in Vero cell line which gives mean satisfactory TCID50/dose (3.34) for used vaccine batches, in addition to clinical examination of vaccinated sheep and also application of challenge test. Sixty susceptible lambs were divided into (10) groups and vaccinated with field and safety doses of (10) different batches of live attenuated vaccine intradermal (I/D) in tail fold while three lambs kept as control. The results showed that lymphocyte proliferation began to increase till reach to its peak (1.312) at 10th day post vaccination then decrease after that with re-increasing after challenge , serological assays results revealed that protective serum antibody titer started at 10th day post vaccination with mean titer (1.6 and 1.99), mean absorbance (1.56 and 2.02) and at three weeks the mean titer (2.35 and 2.61) , mean absorbance (2.43 and 2.51) for NI and ELISA respectively, also all vaccinated lambs showed satisfactory levels of protection against the virulent SPV through challenge test as SID50 more than (2.5) for all batches of vaccine.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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How to Cite This Article

Sonia A Rizk. 2018. \u201cUse of Different Immunoresponse Assays for Evaluation of Live Attenuated Sheep Pox Vaccine in Comparison with Challenge Test\u201d. Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 17 (GJMR Volume 17 Issue G2).

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-G Classification NLMC Code: WA 360

Submission ReceivedDecember 11, 2016
Peer Review Double Blind
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Accepted January 2, 2017
Published January 15, 2017

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January 15, 2018

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Total Score: 104

Country: Egypt

Subject: Global Journal of Medical Research - G: Veterinary Science & Medicine

Authors: Nermeen G. Shafik, Ibrahim M.M, Sonia A. Rizk, Ali A.M (PhD/Dr. count: 0)

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