Kinetic Spectrophotometric Procedure for Investigation of Furosemide Drug in Pharmaceuticals Preparations
A simple, rapid, sensitive, inexpensive, and easy to perform kinetic Spectrophotometric procedure for the investigation of trace quantities of the drug of Furosemide (FRO) as bluk and in the Pharmaceuticals Preparations has been improved. The method was depended on the fashioning of the Schiff’s base by the reaction of aldehyde group present in the 5-sulfo salicylaldehyde reagent and the primary amino group present in Furosemide, which acts as a ligand for the formation of intense colored complex with Co(II) in the acidic medium with maximum absorption at 608nm. the Kinetic Spectrophotometric Method was by using the Fixed Time method for investigation of drug was studied. the law of Beer is applied on the range of concentration between (5 – 100) ppm, the molar absorptivity and the sensitivity of Sandell were 3.9295×104 l.mol-1.cm-1, 0.008 μg.cm-2 subsequently. the detection limit (LOD) was 2.133 μg/ml-1. LOQ was 1.105 μg/ml-1. The perfect circumstances for all colour improvement are portrayed and the suggested procedure has been effectively connected for the investigation of amounts of Furosemide (FRO) in bulk form and in pharmaceutical preparations (tablets, injection sample) The additives and general excipients materials did not affect in the studied method. The statistical comparison between the results that obtained from the reference method gave good agreement.
