Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR In Uganda with Quantitative Real Time RT-PCR on Field Samples

Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR In Uganda with Quantitative Real Time RT-PCR on Field Samples

Hussein Mukasa Kafeero

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Frank Nobert Mwiine

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David Atuhaire Kalenzi

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Sylvester Ochwo

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Ann Nanteza

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Makerere University

Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR In Uganda with Quantitative Real Time RT-PCR on Field Samples

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Abstract

Foot-and-mouth disease (FMD) is a viral disease of Ungulates; both Artiodactyla and Perissodactyla. The mortality rates are low in adult animals but it affects milk yield and international trade. In endemic countries, diagnosis can be based on clinical signs. But these are shared by other vesicular diseases, so a laboratory is needed to confirm the disease. In Uganda the commonly used assays for the laboratory diagnosis of FMD are NSP ELISA and RT-PCR. Serology using ELISA techniques may fail to distinguish between vaccinated and new infection so compromising its sensitivity. The gel passed PCR is involves a lot of advance sample treatment increasing errors due to carry over which also compromises its sensitivity. This work reports comparative the detection of foot-and-mouth virus by NSP ELISA and RT-PCR with real time PCR which was taken as the gold standard. The assays were compared in terms of sensitivity, specificity and disease prevalence and likelihood ratios. A total of 176 cattle were used from which samples that included epithelial tissues (17.05%) and oral swabs (84.09%) were collected from outbreak cases in Eastern Districts of Mbale and Budaka.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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How to Cite This Article

Hussein Mukasa Kafeero. 2016. \u201cComparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR In Uganda with Quantitative Real Time RT-PCR on Field Samples\u201d. Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 16 (GJMR Volume 16 Issue G2).

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GJMR Volume 16 Issue G2

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-G Classification NLMC Code: WC900

Submission ReceivedDecember 15, 2015
Peer Review Double Blind
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Accepted January 5, 2016
Published January 15, 2016

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August 31, 2016

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Total Score: 105

Country: Uganda

Subject: Global Journal of Medical Research - G: Veterinary Science & Medicine

Authors: Hussein Mukasa Kafeero, Frank Nobert Mwiine, David Atuhaire Kalenzi, Sylvester Ochwo, Ann Nanteza (PhD/Dr. count: 0)

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