Synergic Anticancer Activity of Different Extracts of Ziziphusa Mauritiana and Punica Grantum L on the Inhibition of Hela Cell Proliferation and Tumor Growth in EAC Bearing Mice

Synergic Anticancer Activity of Different Extracts of Ziziphusa Mauritiana and Punica Grantum L on the Inhibition of Hela Cell Proliferation and Tumor Growth in EAC Bearing Mice

Somayeh Afsah Vakili

Contact

Arash Parvizi

Contact

Rajiv Gandhi University of Health Sciences

Synergic Anticancer Activity of Different Extracts of Ziziphusa Mauritiana and Punica Grantum L on the Inhibition of Hela Cell Proliferation and Tumor Growth in EAC Bearing Mice

Article Fingerprint

ReserarchID

PDDTMOR6VH

AI TAKEAWAY

Connecting with the Eternal Ground

Abstract

In consideration of the synthetic chemicals chemotherapy has induced obnoxious hazards, there is a ubiquitous tend towards the natural resources, which are remedially potent and affordable for the poor people. Development of phenolic compounds or extracts has become a major area medical research due to their cancer preventive effects through induction apoptosis and prevention of oxidative stress associated cancer. The present scrutiny was designed to investigate the synergic anticancer effect of Punica grantum L and Ziziphus maritiana. The ethanol extract of combination of both plants were found to be the most effective in BSL and trypan blue assay, also stimulated caspase-8 and-9 protease by fold increase in Ac-IETD-pNA. The expression of P 53 in cytoplasm and nuclei and the increase of TdT in the nuclei of carcinoma cells in mice treated while Bcl 2 expression was declined. Consequently, the synergic anticancer activity of ethanol extract of combination of both plants was proved.

References

43 Cites in Article

Reference Format

M Agata,C Fabiano,B Ammar,Alessandra (2009). Flavonoids of Zizyphusjujuba L. and Zizyphus spina-christi (Rhamnaceae) fruits.
Osama Ahmed,Mahmoud Mohamed,Rasha Ahmed,Sayed Ahmed (2009). Synthesis and anti-tumor activities of some new pyridines and pyrazolo[1,5-a]pyrimidines.
M Berenbaum (1970). The mode of action of cytotoxic drug.
A Bhattacharyya,T Choudhuri,S Pal,S Chattopadhyay,G Datta (2003). Apoptogenic effects of black tea on Ehrlich's ascites carcinoma cell.
Chen,Wang (2002). Initiator caspases in apoptotic signalling pathways.
S Chinni,Y Li,S Upadhyay,P Koppolu,F Sarkar (2001). Indole-3-carbinol (l3C) induced cell growth inhibition, G 1 cell cycle arrest and apoptosis in prostate cancer cells.
Athina Damianaki,Efstathia Bakogeorgou,Marilenna Kampa,George Notas,Anastassia Hatzoglou,Simone Panagiotou,Claudia Gemetzi,Elias Kouroumalis,Pierre-Marie Martin,Elias Castanas (2000). Potent inhibitory action of red wine polyphenols on human breast cancer cells.
J Denault,G Salvesen (2002). Caspases: keys in the ignition of cell death.
Min Ding,Rentian Feng,Shiow Wang,Linda Bowman,Yongju Lu,Yong Qian,Vincent Castranova,Bing-Hua Jiang,Xianglin Shi (2006). Cyanidin-3-glucoside, a Natural Product Derived from Blackberry, Exhibits Chemopreventive and Chemotherapeutic Activity.
P Fresco,F Borges,C Diniz,M Marques (2006). New insights on the anticancer properties of dietary polyphenols.
Yaron Fuchs,Hermann Steller (2000). Programmed Cell Death in Animal Development and Disease.
G Galati,,S Teng,,M Moridani,,T Chan,,P O'brien, (2000). Cancer Chemoprevention and Apoptosis Mechanisms Induced by Dietary Polyphenolics.
Anitha Genupur,Josepha Jesu,Narasimhan Srinivasan,Anand Kamalakaran,Rajan Sundar (2006). Synthesis and cytotoxicity of novel isomeric C-seco limonoids.
C Gerhauser (2008). Cancer chemopreventive potential of apples, apple juice, and apple components.
P Giannakakou,R Robey,T Fojo,M Blagosklonny (2001). Low concentrations of paclitaxel induce cell type-dependent p53, p21 and G 1 / G 2 arrest instead of mitotic arrest: molecular determinants of paclitaxel-induced cytotoxicity.
H Goa,Y Zhou (2005). Inhibitory effect of picroside II on hepatocyte apoptosis.
Michael Hengartner (2000). The biochemistry of apoptosis.
Jun-Ya U Yasuhiro,T Arjun,H Quan,L Qui,K Yuko,H Ikuo,S Shigetoshi,K (2002). Antiproliferative Activity of Vietnamese Medicinal Plants.
Marilenna Kampa,Anastassia Hatzoglou,George Notas,Athina Damianaki,Efstathia Bakogeorgou,Claudia Gemetzi,Elias Kouroumalis,Pierre-Marie Martin,Elias Castanas (2000). Wine Antioxidant Polyphenols Inhibit the Proliferation of Human Prostate Cancer Cell Lines.
S Kasibhatla,B Tseng (2003). Minireview: Why target apoptosis in cancer treatment.
N Kumar,S Shruti,C Ajanta,B Naganjaneyulu,K Rama,Partha (2014). Role of isothiocyanate conjugate of pterostilbene on the inhibition of MCF-7 cell proliferation and tumor growth in Ehrlich ascitic cell induced tumor bearing mice.
Geeta Lala,Minnie Malik,Cuiwei Zhao,Jian He,Youngjoo Kwon,M Giusti,Bernadene Magnuson (2006). Anthocyanin-Rich Extracts Inhibit Multiple Biomarkers of Colon Cancer in Rats.
J Lambert,C Yang (2003). Cancer chemopreventive activity and bioavailability of tea and tea polyphenols.
B Meyer,N Ferrigni,J Putnam,L Jacobsen,D Nichols,J Mclaughlin (1982). Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents.
S Nagata (2000). Apoptotic DNA fragmentation.
S Nichenametla,T Taruscio,D Barney,J Exon (2006). A review of the effects and mechanisms of polyphenolics in cancer.
D Nijhawan,I Budihardjo,S Srinivasula,M Ahmad,E Alnemri,Wang (1997). Cytochrome-c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.
A Pain,S Samanta,S Dutta (2003). Evaluation of naphthalymustine, a nitrogen mustard derivative of naphthalimide as a rationally designed anticancer agent.
Sonia Ramos (2007). Effects of dietary flavonoids on apoptotic pathways related to cancer chemoprevention.
Sonia Ramos,Mario Alía,Laura Bravo,Luis Goya (2005). Comparative Effects of Food-Derived Polyphenols on the Viability and Apoptosis of a Human Hepatoma Cell Line (HepG2).
M Saluja,B Sangameswaran,I Hura,S Ajay,S Gupta,M Chaturvedi (2011). In-vitro cytotoxic activity of leaves of Madhuca longifolia against Ehrlich Ascites carcinoma (EAC) cell lines.
N Seeram,L Adams,Y Zhang,R Lee,D ; ) Sand,H Scheuller,D Heber (2006). Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro.
A Seraste,K Pulkki (2000). Morphologic and biochemical hallmarks of apoptosis.
M Sharrif,H Hamed (2012). Chemical composition of the plant Punicagranatum L and its effect on heart and cancer.
Djk Silva,P Sousa,E Andrade,Maia Jg (2007). Antioxidant capacity and cytotoxicity of essential oil and methanol extract of Aniba canelilla (H.B.K.) Mez.
Igor Sobenin,Vladimir Tertov,Alexander Orekhov (1998). Optimization of the assay for sialic acid determination in low density lipoprotein.
S Thomasset,N Teller,H Cai,D Marko,D Berry,W Steward,A Gescher (2009). Do anthocyanins and anthocyanidins, cancer chemopreventive pigments in the diet, merit development as potential drugs?.
C Thompson (1995). Apoptosis in the pathogenesis and treatment of disease.
J Weisburg,D Weissman,T Sedaghat,H Babich (2004). In vitro cytotoxicity of epigallocatechin gallate and tea extracts to cancerous and normal cells from the human oral cavity.
A Wyllie (1980). Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation.
C Yang,P Maliakal,X Meng (2002). Inhibition of carcinogenesis by tea.
Guoying Zhang,Yutaka Miura,Kazumi Yagasaki (1999). Effects of green, oolong and black teas and related components on the proliferation and invasion of hepatoma cells in culture.
Y Zhang,N Seeram,R Lee,L Feng,D Heber (2008). Isolation and identification of strawberry phenolics with antioxidant and human cancer cell antiproliferative properties.

Download References

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Somayeh Afsah Vakili. 2017. \u201cSynergic Anticancer Activity of Different Extracts of Ziziphusa Mauritiana and Punica Grantum L on the Inhibition of Hela Cell Proliferation and Tumor Growth in EAC Bearing Mice\u201d. Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine GJMR-B Volume 16 (GJMR Volume 16 Issue B4).

More Citation Formats

Select Citation Style:

Download Citation

GJMR Volume 16 Issue B4

Explore Journals Explore Volume Read This Issue

Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

Not Found

Classification

GJMR-B Classification NLMC Code: QZ 20.5

Submission ReceivedDecember 14, 2015
Peer Review Double Blind
Handling Editor
Accepted December 31, 2015
Published January 15, 2016

Version of record

v1.2

Issue date

February 2, 2017

Language

Experiance in AR

Explore published articles in an immersive Augmented Reality environment. Our platform converts research papers into interactive 3D books, allowing readers to view and interact with content using AR and VR compatible devices.

View in VR

Read in 3D

Your published article is automatically converted into a realistic 3D book. Flip through pages and read research papers in a more engaging and interactive format.

View in 3D

Article Matrices

Total Score: 102

Country: India

Subject: Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine

Authors: Somayeh Afsah Vakili, Arash Parvizi (PhD/Dr. count: 0)

View Count (all-time): 185

Total Views (Real + Logic): 3695

Total Downloads (simulated): 1854

Publish Date: 2017 02, Thu

Monthly Totals (Real + Logic):

Month 1: 41 views
Month 2: 48 views
Month 3: 55 views
Month 4: 64 views
Month 5: 22 views
Month 6: 39 views
Month 7: 29 views
Month 8: 51 views
Month 9: 52 views
Month 10: 27 views
Month 11: 37 views
Month 12: 26 views
Month 13: 30 views
Month 14: 17 views
Month 15: 33 views
Month 16: 27 views
Month 17: 21 views
Month 18: 20 views
Month 19: 39 views
Month 20: 41 views
Month 21: 32 views
Month 22: 27 views
Month 23: 21 views
Month 24: 44 views
Month 25: 28 views
Month 26: 40 views
Month 27: 20 views
Month 28: 11 views
Month 29: 35 views
Month 30: 31 views
Month 31: 28 views
Month 32: 21 views
Month 33: 37 views
Month 34: 29 views
Month 35: 28 views
Month 36: 22 views
Month 37: 29 views
Month 38: 34 views
Month 39: 40 views
Month 40: 37 views
Month 41: 44 views
Month 42: 39 views
Month 43: 27 views
Month 44: 24 views
Month 45: 34 views
Month 46: 40 views
Month 47: 39 views
Month 48: 31 views
Month 49: 25 views
Month 50: 27 views
Month 51: 36 views
Month 52: 37 views
Month 53: 37 views
Month 54: 16 views
Month 55: 32 views
Month 56: 18 views
Month 57: 31 views
Month 58: 32 views
Month 59: 29 views
Month 60: 22 views
Month 61: 42 views
Month 62: 38 views
Month 63: 17 views
Month 64: 36 views
Month 65: 38 views
Month 66: 22 views
Month 67: 18 views
Month 68: 23 views
Month 69: 26 views
Month 70: 41 views
Month 71: 35 views
Month 72: 22 views
Month 73: 27 views
Month 74: 15 views
Month 75: 35 views
Month 76: 30 views
Month 77: 21 views
Month 78: 26 views
Month 79: 26 views
Month 80: 26 views
Month 81: 35 views
Month 82: 33 views
Month 83: 31 views
Month 84: 15 views
Month 85: 29 views
Month 86: 33 views
Month 87: 31 views
Month 88: 36 views
Month 89: 24 views
Month 90: 33 views
Month 91: 21 views
Month 92: 42 views
Month 93: 26 views
Month 94: 14 views
Month 95: 36 views
Month 96: 36 views
Month 97: 35 views
Month 98: 36 views
Month 99: 20 views
Month 100: 36 views
Month 101: 43 views
Month 102: 29 views
Month 103: 46 views
Month 104: 47 views
Month 105: 25 views
Month 106: 25 views
Month 107: 45 views
Month 108: 38 views
Month 109: 51 views
Month 110: 32 views
Month 111: 49 views
Month 112: 36 views

Total Views: 3695

Total Downloads: 1854

2026 Trends

Our website is actively being updated, and changes may occur frequently. Please clear your browser cache if needed. For feedback or error reporting, please email [email protected]