REVERSE PHASE aHIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ULTRA VIOLET SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF RALTEGRAVIR POTASSIUM IN BULK

Dr. T.SUDHA

REVERSE PHASE aHIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ULTRA VIOLET SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF RALTEGRAVIR POTASSIUM IN BULK

Dr. T.SUDHA *

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REVERSE PHASE aHIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ULTRA VIOLET SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF RALTEGRAVIR POTASSIUM IN BULK

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Abstract

A High Performance Liquid Chromatographic (method A) and Ultra Violet spectrophotometric (method B) method were developed and validated for quantitative determination of Raltegravir potassium. The different analytical performance parameters such as linearity, precision, accuracy, limit of detection (LOD), limit of Quantification (LOQ) were determined according to International Conference on Harmonization (ICH) Q2B guidelines. Reverse phase High Performance Liquid Chromatography (method A) and Ultra Violet (method B) were developed for the determination of Raltegravir potassium in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved on Symmetry C18 (4.6 x 150mm, 5 μm XTerra) column using a mixture of phosphate buffer (pH 3.0): Methanol (45:55% v/v) as the mobile phase at a flow rate 0.6 mL min-1. The Ultra Violet spectrophotometric determination was performed at 219 nm. The Linearity of the calibration curves for the analyte in the desired concentration range is good (r2 = 0.999) by both High Performance Liquid Chromatography (method A) and Ultra Violet (method B) spectroscopic method. Both methods (A&B) were accurate and precise with recoveries in the range of 98-100 % and percentage relative standard deviation (RSD less than 2%). The proposed methods were highly sensitive, accurate, and precise and hence was successfully applied for the reliable quantification of Active Pharmaceutical Ingredient content in the commercial formulation.

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Dr. T.SUDHA. 1970. "REVERSE PHASE aHIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ULTRA VIOLET SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF RALTEGRAVIR POTASSIUM IN BULK". Global Journal of Medical Research GJMR Volume 11 (GJMR Volume 11 Issue 2).

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GJMR Volume 11 Issue 2

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

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Classification

GJMR Classification NLMC Code: QV 277

Submission ReceivedMay 20, 2011
Peer Review Double Blind
Handling Editor
Accepted June 19, 2011
Published July 4, 2011

Version of record

v1.2

Issue date

May 20, 2011

Language

English

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Article Matrices

Total Score: 147

Country: India

Subject: Global Journal of Medical Research

Authors: Dr. T.SUDHA, T.RAGHUPATHI (PhD/Dr. count: 1)

View Count (all-time): 266

Total Views (Real + Logic): 6277

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Publish Date: 2011 07, Mon

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Total Views: 6.3K

Total Downloads: 267

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