Direct antisperm antibody examination of infertile men
The study was conducted to elucidate the correlation between direct antisperm antibody (ASA) test and some semen characters of infertile men at the Institute of Embryo Researches and Infertility Treatment at Al-Nahrain University from March 2009 to June 2010. Microscopic and macroscopic semen analysis, Direct Sperm MAR IgA test and Direct Sperm MAR IgG test were performed on semen samples. Forty-two men were included in the study, of whom, all underwent direct sperm MAR IgA test, but due to observer bias, only 36 Direct sperm MAR IgG tests were included in the study. There was a significant correlation between suspected IgA and viscosity (r=0.65, p=0.02 ); suspected IgA and pH (r=0.42, p=0.04 ); and suspected IgG and liquefaction time (r=0.44, p=0.04). A significant negative correlation was found between suspected IgG and progressive sperm motility (r=-0.44, p=0.04); suspected IgG and suspected IgA with morphologically normal sperm percentage (r=-0.65, p=0.03 and r=-0.43, p= 0.04, respectively); suspected IgG and sperm agglutination (r=- 0.54, p=0.03). This study, recommends the use of Direct tests instead of the indirect assays in andrology laboratories in Iraq.
