Mediated Liposome for Gene Delivery to Mice Brain Part I.  Design and Characterization of Liposome-DNA Complexes

Mediated Liposome for Gene Delivery to Mice Brain Part I. Design and Characterization of Liposome-DNA Complexes

E.S. Ghaly

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J. Wang

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University of Puerto Rico, Medical Sciences Campus

Mediated Liposome for Gene Delivery to Mice Brain Part I. Design and Characterization of Liposome-DNA Complexes

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Abstract

The purpose of this research is to develop a novel liposome-mediated system for delivery of expression plasmid into specific regions in the rat brain. Complexes of plasmid DNA and different liposome were prepared in phase 1 of the study. The composition, method of preparation were varied and the physico-chemical characterization of the different systems were investigated two different methods of preparation were used, in the first method the liposome were prepared simultaneously with the DNA entrapped into the liposome and in the second method, the liposome were prepared first and then complexes with the DNA were performed. The liposome formulations were composed of DOTAP: Cholesterol; and DC-Chol: DOPE and different lipid helpers. The particle size of liposomes prepared with DNA entrapped into the liposome was larger than those prepared with liposome-DNA complexation. All liposome formulations were spherical, uniform in size and have smooth surface. In vitro DNase digestion experiments demonstrated that liposome protects 60-80% plasmid DNA from DNase digestion. The plasmid: DNA lmediated liposome can be widely prepared, have less risk than the use of viral vectors, can protect DNA from DNase digestion, none toxic and therefore can be used repeatedly in vivo.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

E.S. Ghaly. 2013. \u201cMediated Liposome for Gene Delivery to Mice Brain Part I. Design and Characterization of Liposome-DNA Complexes\u201d. Global Journal of Medical Research - K: Interdisciplinary GJMR-K Volume 13 (GJMR Volume 13 Issue K2).

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GJMR Volume 13 Issue K2

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

gene delivery gene expression mediated liposomes plasmid-DNA expression targeting targeting to mice brain

Submission ReceivedDecember 16, 2012
Peer Review Double Blind
Handling Editor
Accepted January 1, 2013
Published January 15, 2013

Version of record

v1.2

Issue date

May 2, 2013

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Article Matrices

Total Score: 132

Country: United States

Subject: Global Journal of Medical Research - K: Interdisciplinary

Authors: J. Wang, E.S. Ghaly (PhD/Dr. count: 0)

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Publish Date: 2013 05, Thu

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Total Views: 4820

Total Downloads: 2420

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