Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

Kashinath R.T. Msc Biochemistry, Ph.D. Biochemistry (Medical Faculty), FICS

Contact

Nagendra .S.

Contact

Rudrappa. G.

Contact

Srinivas.S.

Contact

Rajiv Gandhi University of Health Sciences

Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

Article Fingerprint

ReserarchID

2EQCV

Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects Banner

AI TAKEAWAY

Connecting with the Eternal Ground

Abstract

Generally, in fasting state, gluconeogenesis accounts for about 30% of overall hepatic glucose output, the increase in type 2 diabetic subjects may be much more than this level. There is a close relationship between glucose metabolism and amino acid metabolism which is established through transamination reactions. The key amino acid -keto acid pairs involved in transamination reactions are glutamateα ketoglutarate, aspartate -oxaloacetate and pyruvate -alanine. A study was undertaken to assess the plasma levels of total amino acids, glutamate and alanine in type 2 diabetic subjects. A blood sample (5ml) with heparin as an anticoagulant was collected in the fasting state, from each of the selected normal as well as diabetic subjects. The separated plasma samples were employed for the estimation of total amino acid nitrogen levels, as well as for the estimation of plasma alanineand glutamate levels. The results indicate a significant rise in the plasma levels of total amino acids, alanine & glutamate in type-2 diabetic subjects as well as a significant increase in plasma alanine levels in diabetics of 30-40 yrs age as compared to diabetics of 41-60yrs of age. These findings suggest an increased availability of glucogenic amino acid precursors for glucose formation may be due to lack of proteolytic suppression of insulin.

References

19 Cites in Article

Reference Format

Agostino Consoli,Nurjahan Nurjahan,J James,Reilly,M Dennis,John Beir,Gerich (1990). Mechanism of increased gluconeogenesis in noninsulin dependent diabetes mellitus.
J Best,R Judzewitsch,M Pfeifer,J Beard,J Halter,D Porte (1982). The effect of chronic sulfonylurea therapy on hepatic glucose production in non-insulin-dependent diabetes.
A Consoli,N Nurjhan,F Capani,J Gerich (1989). Predominant role of gluconeogenesis in increased hepatic glucose production in NIDDM.
R Defronzo,R Gunnarsson,O Bjorkman,J Olsson M & Wahren (1985). Effects of insulin on peripherial and splanchinic glucose metabolism in non-insulin dependent (type-2) diabetes mellitus.
R Defronzo,D Simonson,E Ferrannini (1982). Hepatic and peripheral insulin resistance: A common feature of Type 2 (non-insulin-dependent) and Type 1 (insulin-dependent) diabetes mellitus.
R Defranzo,A Golay,J Felbar (1985). Glucose and lipid metabolism in obesity and diabetes mellitus in "Substrate and energy metabolism.
R Bell,P Marsh,H Hanson,J Rizza,R (1986). Post-Prandial hyperglycemia in patients with non insulin dependent diabetes mellitus. Role of hepatic and extra hepatic tissues.
Folin (1965). cited in "Hawk's physiological chemistry.
P Fao,G Galasino,Costa (1955). Unknown Title.
R Fulks,J Li,A Goldberg (1975). Effects of insulin, glucose, and amino acids on protein turnover in rat diaphragm..
J Gerich,A Mitrakou,D Kelley,L Mandarino,N Nurjhan,J Reilly,T Jenssen,T Veneman,A Consoli (1990). Contribution of impaired muscle glucose clearance to reduced postabsorptive systemic glucose clearance in NIDDM.
Joseph Goldstein,Michael Brown,Richard Anderson,David Russell,Wolfgang Schneider (1995). Receptor-Mediated Endocytosis: Concepts Emerging from the LDL Receptor System.
R Garcia,V Gazola,H Barrena,E Hartmann,J Berti,M Toyama,A Boschero,E Carneiro,F Manso,R Bazotte (2007). Blood amino acids concentration during insulin induced hypoglycemia in rats: the role of alanine and glutamine in glucose recovery.
L Jefferson,D Rannels,B Munger,Morgan (1974). Insulin in the regulation of protein turnover in the heart and skeletal muscle.
R Kries Berg (1972). Glucose -Lactate interrelations in man.
K Loundholm,S Edstrom,L Ekman,I Karlerg,P Walker,T Schersten (1981). Protein degradation in human skeletal muscle tissue, the effect of insulin, leucine, amino acid, ions.
Richard J Block,L Emmett Durram & Guntar Zweig cited in "A Manual of paper Chromatography and paper electrophoresis.
Earl Sutherland,Carl Cori (1951). EFFECT OF HYPERGLYCEMIC-GLYCOGENOLYTIC FACTOR AND EPINEPHRINE ON LIVER PHOSPHORYLASE.
P Vishweshwaran,K Subramanyan (1970). Unknown Title.

Download References

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Kashinath R.T.. 2014. \u201cPlasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects\u201d. Global Journal of Medical Research - K: Interdisciplinary GJMR-K Volume 14 (GJMR Volume 14 Issue K1).

More Citation Formats

Select Citation Style:

Download Citation

GJMR Volume 14 Issue K1

Explore Journals Explore Volume Read This Issue

Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

alanine amino acids gluconeogenesis glutamate

Submission ReceivedDecember 11, 2013
Peer Review Double Blind
Handling Editor
Accepted January 5, 2014
Published January 15, 2014

Version of record

v1.2

Issue date

March 13, 2014

Language

Experiance in AR

Explore published articles in an immersive Augmented Reality environment. Our platform converts research papers into interactive 3D books, allowing readers to view and interact with content using AR and VR compatible devices.

View in VR

Read in 3D

Your published article is automatically converted into a realistic 3D book. Flip through pages and read research papers in a more engaging and interactive format.

View in 3D

Article Matrices

Total Score: 104

Country: Unknown

Subject: Global Journal of Medical Research - K: Interdisciplinary

Authors: Kashinath R.T., Nagendra .S., Rudrappa. G., Srinivas.S. (PhD/Dr. count: 0)

View Count (all-time): 179

Total Views (Real + Logic): 4716

Total Downloads (simulated): 2374

Publish Date: 2014 03, Thu

Monthly Totals (Real + Logic):

Month 1: 27 views
Month 2: 36 views
Month 3: 36 views
Month 4: 41 views
Month 5: 39 views
Month 6: 22 views
Month 7: 27 views
Month 8: 31 views
Month 9: 40 views
Month 10: 50 views
Month 11: 32 views
Month 12: 28 views
Month 13: 33 views
Month 14: 17 views
Month 15: 23 views
Month 16: 45 views
Month 17: 12 views
Month 18: 25 views
Month 19: 41 views
Month 20: 45 views
Month 21: 25 views
Month 22: 40 views
Month 23: 20 views
Month 24: 36 views
Month 25: 21 views
Month 26: 38 views
Month 27: 27 views
Month 28: 24 views
Month 29: 12 views
Month 30: 47 views
Month 31: 27 views
Month 32: 30 views
Month 33: 34 views
Month 34: 17 views
Month 35: 33 views
Month 36: 11 views
Month 37: 44 views
Month 38: 35 views
Month 39: 31 views
Month 40: 34 views
Month 41: 32 views
Month 42: 41 views
Month 43: 45 views
Month 44: 28 views
Month 45: 35 views
Month 46: 24 views
Month 47: 39 views
Month 48: 26 views
Month 49: 42 views
Month 50: 42 views
Month 51: 17 views
Month 52: 30 views
Month 53: 32 views
Month 54: 20 views
Month 55: 43 views
Month 56: 18 views
Month 57: 32 views
Month 58: 44 views
Month 59: 15 views
Month 60: 15 views
Month 61: 40 views
Month 62: 23 views
Month 63: 35 views
Month 64: 21 views
Month 65: 20 views
Month 66: 24 views
Month 67: 43 views
Month 68: 29 views
Month 69: 35 views
Month 70: 35 views
Month 71: 18 views
Month 72: 28 views
Month 73: 24 views
Month 74: 39 views
Month 75: 20 views
Month 76: 40 views
Month 77: 34 views
Month 78: 32 views
Month 79: 32 views
Month 80: 28 views
Month 81: 42 views
Month 82: 15 views
Month 83: 33 views
Month 84: 36 views
Month 85: 16 views
Month 86: 25 views
Month 87: 39 views
Month 88: 43 views
Month 89: 43 views
Month 90: 36 views
Month 91: 42 views
Month 92: 28 views
Month 93: 24 views
Month 94: 26 views
Month 95: 24 views
Month 96: 31 views
Month 97: 30 views
Month 98: 34 views
Month 99: 24 views
Month 100: 33 views
Month 101: 29 views
Month 102: 29 views
Month 103: 22 views
Month 104: 38 views
Month 105: 27 views
Month 106: 36 views
Month 107: 25 views
Month 108: 35 views
Month 109: 22 views
Month 110: 15 views
Month 111: 34 views
Month 112: 11 views
Month 113: 20 views
Month 114: 41 views
Month 115: 35 views
Month 116: 30 views
Month 117: 30 views
Month 118: 24 views
Month 119: 45 views
Month 120: 46 views
Month 121: 33 views
Month 122: 16 views
Month 123: 42 views
Month 124: 25 views
Month 125: 32 views
Month 126: 26 views
Month 127: 40 views
Month 128: 30 views
Month 129: 30 views
Month 130: 26 views
Month 131: 48 views
Month 132: 27 views
Month 133: 37 views
Month 134: 14 views
Month 135: 22 views
Month 136: 33 views
Month 137: 36 views
Month 138: 52 views
Month 139: 54 views
Month 140: 29 views
Month 141: 35 views
Month 142: 41 views
Month 143: 23 views
Month 144: 61 views
Month 145: 34 views
Month 146: 37 views
Month 147: 46 views

Total Views: 4716

Total Downloads: 2374

2026 Trends

Our website is actively being updated, and changes may occur frequently. Please clear your browser cache if needed. For feedback or error reporting, please email [email protected]