Study on Cell Concentration in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural by products

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M. Sathiyamoorthy
M. Sathiyamoorthy
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Dr. S.Theneshkumar
Dr. S.Theneshkumar

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Study on Cell Concentration in the Production and Optimization of High Temperature Alkaline  I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural by products

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Abstract

Production of α-amylase enzyme by Bacillus Lichenoformis using stirred tank fermentor (BIOSTAT -E) was carried out. The strain was obtained from National Chemical Laboratory, Pune, India. Corn starch is used as the substrate. The enzyme production was studied by changing the various parameters like temperature, pH, rpm and substrate concentration. The biomass cell concentration shows maximum at a temperature of 35 0 C -37 0 C, pH 8 and 300 rpm. The maximum enzyme production was achieved, for 1% concentration of cornstarch at 35 0 C and pH 8 using the fermentation medium contains yeast extract and peptone and the cell concentration was found to be 2.882 gm dry weight/lit. Since the cost of yeast extract and peptone is very high, so the further work was done using some low cost carbon and nitrogen sources like defatted cotton seed, defatted soya flour and mustard seed which are extracted from agricultural byproducts.

References

11 Cites in Article
  1. A Gandhi,L Kjaergaard (1975). Effect of CO2 on the formation of α -amylase by Bacillus subtillis growing in continuous and batch cultures.
  2. Griffon Fogarty,A Joyce (1974). Bacillus proteolyticus species which produced an alkaline protease.
  3. H Rehm,G Read (1987). a, Enzyme technology.
  4. J Jayaraman (1981). Laboratory manual in Biochemistry.
  5. James Bailey,David Ollis (1986). Biochemical engineering fundamentals.
  6. H Martha,Leonard Oseley,Keay (1970). Purification and characterization of the α -amylase of Bacillus subtillis NRRL B3411.
  7. Peter Stanbury,Allan Whitaker,Stephen Hall (1984). Fermentation Economics.
  8. Pratima Bajpai,Pramod Bajpai (1989). High‐temperature alkaline α‐amylase from <i>Bacillus iicheniformis</i> TCRDC‐B13.
  9. Pratima Bajpai,Umender Sharma (1989). Production of α -amylase in a low cost medium by Bacillus lichenoformis.
  10. Seung-Hyeon Moon,Satish Parulekar (1993). A parametric study of x-amylase production in Batch, Fed batch and continuous suspension culture of Bacillus firmus.
  11. Yong Hee,Lower (1990). Production of alkaline protease by Bacillus lichenoformis in an aqueous two phase system.

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

M. Sathiyamoorthy. 2014. \u201cStudy on Cell Concentration in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural by products\u201d. Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 14 (GJSFR Volume 14 Issue G2): .

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Issue Cover
GJSFR Volume 14 Issue G2
Pg. 33- 39
Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Version of record

v1.2

Issue date

July 24, 2014

Language
en
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Published Article

Production of α-amylase enzyme by Bacillus Lichenoformis using stirred tank fermentor (BIOSTAT -E) was carried out. The strain was obtained from National Chemical Laboratory, Pune, India. Corn starch is used as the substrate. The enzyme production was studied by changing the various parameters like temperature, pH, rpm and substrate concentration. The biomass cell concentration shows maximum at a temperature of 35 0 C -37 0 C, pH 8 and 300 rpm. The maximum enzyme production was achieved, for 1% concentration of cornstarch at 35 0 C and pH 8 using the fermentation medium contains yeast extract and peptone and the cell concentration was found to be 2.882 gm dry weight/lit. Since the cost of yeast extract and peptone is very high, so the further work was done using some low cost carbon and nitrogen sources like defatted cotton seed, defatted soya flour and mustard seed which are extracted from agricultural byproducts.

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Study on Cell Concentration in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural by products

M. Sathiyamoorthy
M. Sathiyamoorthy
Dr. S.Theneshkumar
Dr. S.Theneshkumar

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