Screening of Therapeutic Potential and Compounds of Endemic Nepeta pilinux P.H. Davis in Kew Bull. from Şanlıurfa
Nepeta pilinux P.H. Davis in Kew Bull. was recently recorded as an endemic species in Birecik, Şanlıurfa. The essential oils were obtained from air-dried aerial parts by hydrodistillation and their composition was investigated using GC-FID and GC/MS. Determination of antioxidant capacity, and urease and xanthine oxidase inhibitions of the methanolic extracts were performed with HPLC-DAD and spectrophotometry. 34 compounds were identified constituting 89.2% of the total essential oil compounds. The major components were determined as T-cadinol (31.1%), γ-muurolene (14.4%) and 14-nor-cadin-5-en-4-one isomer A (11.0%) in the oil. Mainly rosmarinic acid, chlorogenic acid, and caffeic acid derivatives were quantified together with apigenin, luteolin and tangeretin derivatives in the extracts by HPLC-DAD. The total phenolics of the extract from leaf and flower parts, 50.81 mg GAE.g-1, was higher than the extract from stem part, and the radical scavenging activity of this extract was also stronger. While, the leaf and flower extract had significant urease and xanthine oxidase inhibitory activities (62.47 and 48.48 µg.mL-1), stem extract had low inhibition on both enzymes.
