Bio
Bilal Ahmed is affiliated with the University of Agriculture Faisalabad, Pakistan, specifically with the Department of Chemistry and Biochemistry. He has engaged in research related to drug metabolism and molecular biology and holds a Ph.D. degree.
Experience
Editors Role
Reviewer
GJMR
2014 -Research
Comparative Analysis of Antiglycation Capacity of Aqueous and Methanolic Extracts of Vegetables
Glycation is a reaction between amino group of blood proteins and reducing sugars in vitro conditions which are involved in a number of pathologies and disease states including Alzheimer’s and diabetes. Equal concentration of different inhibitor extracts (sweet potato, turnip and methi) and glucose were used. Eight combinations of each extract were made and all these were placed at 37oC for five weeks incubation. Human normal plasma was used as a protein source. Glycation was analyzed by Thiobarbituric acid (TBA) technique which results that aqueous and methanol extracts of sweet potato and turnip showed no inhibition of non-enzymatic glycation but act as activator of reaction while aqueous extract of methi showed maximum inhibition of non-enzymatic glycation in 5th week of incubation and for methanol extract inhibition was maximum in 3rd week of incubation. In all extracts of three vegetables, extracts of methi were more effective against non-enzymatic glycation. These findings suggest that in future methi can be used for lowering glucose level in the body as it is efficient in lowering the glycation level in different conditions when level of glucose is high.
Urinary Excretion and Renal Clearance of Nimesulide in Male Volunteers
Drugs are eliminated from the body either unchanged as the parent drug or as metabolites. Nimesulide drug belongs to a wide class of medicines called antiinflammatory nonsteroidal drugs (NSAID). NSAIDs are well known for their antipyretic, anti-inflammatory, and analgesic properties. Renal clearance of nimesulide correlated with creatinine clearance. The present research work was planed to determine urinary excretion and renal clearance of nimesulide after its oral administration. Blood and urine samples of the human male volunteers (n=10), after the oral administration of drug, were collected at predetermined time intervals. The concentration of the nimesulide in urine and plasma of male volunteers was determined by HPLC. Statistical analysis was performed in order to determine the significance of the results.
Urinary Excretion and Renal Clearance of Allopurinol in Male Gout Patients
Drugs removed from the body either without changing from its original form or in the form of its metabolite. Allopurinol drug decreases uric acid level in blood and it is used for the treatment of gout and tumor lysis syndrome. Allopurinol and its active metabolite oxipurinol stop the function of xanthine oxidase which forms uric acid from xanthine and hypoxanthine. In this study a quantitative assay using high-performance liquid chromatography (HPLC) with UV-detection was used as a method for quantification of allopurinol and oxipurinol in human serum and urine samples of gout patients after allopurinol administration. The urinary excretion and renal clearance was determined in male gout patients. Blood and urine samples of the human male patients of gout (n=10) after the oral administration of 300mg drug were taken at different time intervals. Results of this study show that there is slow metabolism of allopurinol inside body due to its extensive bonding with blood proteins. Statistical analysis was performed by expressing all the data as mean and ± standard error of mean. The effect of pH and diuresis on renal clearance of allopurinol was studied by regression analysis.
