Research
Serotyping of Salmonella Enterica Isolated from Broiler Chicks in an Outbreak in Sudan
Aims: This study investigates the first emergence of S almonella outbreaks among 8.000 broiler chicks, of the ‘ Ross’ breed, in Sudan. Mortality was observed in 25% of the t otal chicks; therefore, samples were taken for culturing, i dentification and characterization of the causative agent. Study design: A total of 52 random samples, out of 2000 i nfected broiler chicks, were cultured and identified. The study was undertaken in the laboratories of the Veterinary Research Institute, Ministry of animal resources and fisheries, Khartoum as well as Department of Botany, Faculty of Science, University of Khartoum, Sudan during March 2014- December 2014. Results: All of the 52 isolates have shown colony characteristic t ypical to Salmonella spp. Result of the Vitek2 compact system s howed that isolate was typical Salmonella enterica. The p rimer pairs targeting invA and hilA genes successfully a mplified the extracted DNA giving the specific amplicons.
Bacteria Associated with Pneumonia in Camels (Camelus Dromedarius) in the Sudan and Sensitivity of some Isolates to Antibiotics using Vitek 2 Compact.
Aims: This study is to isolate and characterized the bacterial isolates associated with pneumonia in Sudanese camels using different methods and test the sensitivity of some isolates to antibiotics. Study design: A total of 800 affected lung samples were collected from 400 camels (Camelus dromedarius ) of different ages, seasons and part of the Sudan. Place and Duration of Study: The study was undertaken in the laboratories of the Veterinary Research Institute, Ministry of animal resources and fisheries during 2009 to 2013. Methodology: The bacterial isolates were characterized by three different methods: the conventional, Api kits and automated Vitek 2 Compact System. Fourteen of the Gram negative and 11 of the Gram positive bacterial isolates were tested for sensitivity to antibiotics by the Vitek 2 Compact using a sensitivity card which contained 20-21 antibiotics.
