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A Rapid and Efficient Method for Purification of Peroxidase from Opuntia-ficus indica Stem with Decolorization Effect

§ Alanya Alaaddin Keykubat University

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A Rapid and Efficient Method for Purification of Peroxidase from Opuntia-ficus indica Stem with Decolorization Effect

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Abstract

Peroxidase enzyme was purified from Opuntia ficusindica stem for decolorization some synthetic dyes including congo red, methyl red, indigo carmine, crystal violet and ponceau red by TPP. For the purification of the enzyme, the stem of a fresh plant was first homogenized by using phosphate buffer, and subsequently, supernatant was filtered. The homogenate was mixed with ammonium sulfate at room temperature, and t-butanol was added. The intermediate phase was separated and optimized according to different process parameters as homogenate/tbutanol ratio (1:0.5), ammonium sulfate concentration (40%), pH (8), temperature (30°C) and values of K M (4.8 mM) and V max (0,005 U/mL.min) with 2, 25-fold purification and 106, 64% recovery. The molecular weight of partitioned enzyme was found about 28 kDa compared with protein standard. TPP was found to be an easy and effective applicable technique with utilization for extraction and purification of peroxidase enzyme and the purified enzyme decolorized the tested synthetic dyes, successfully. The most important advantage of the method is a high recovery of peroxidase enzyme without requirement any column.

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Fatih Uckaya, Meryem Uckaya. 2018. "A Rapid and Efficient Method for Purification of Peroxidase from Opuntia-ficus indica Stem with Decolorization Effect". Global Journal of Science Frontier Research - B: Chemistry GJSFR-B Volume 18 (GJSFR Volume 18 Issue B3).

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords
Classification
GJSFR-B Classification FOR Code: 030599
Version of record

v1.2

Issue date
September 5, 2018

Language
English
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A Rapid and Efficient Method for Purification of Peroxidase from Opuntia-ficus indica Stem with Decolorization Effect

Fatih Uckaya
Fatih Uckaya Alanya Alaaddin Keykubat University
Meryem Uckaya
Meryem Uckaya <p>Alanya Alaaddin Keykubat University</p>