Alternatives to Fetal Bovine Serum in Different Cell Cultures

Alternatives to Fetal Bovine Serum in Different Cell Cultures

Safaa A. Warda

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Hossam A. Fahmy

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Fatma F. Warda

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Alternatives to Fetal Bovine Serum in Different Cell Cultures

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Abstract

Culture of animal cells is a key operation in bioscience at which fetal bovine serum is the most widely used. Other new alternatives were examined for growth of cell culture as newly born calf serum (NBCS), goat serum (GS) and Equine platelet lysate (EPL). Cell growth and viability was investigated in target cells (Vero-MDBK) using different concentration of alternatives. In general the platelet lysate medium 7 -10 % (EPL) supported cell growth and maintained viabilities comparable or superior to (NBCS). Goat serum exihbit heterologous cells in the first passage then the growth were similar to newly born calf serum with adding low concentration % of (GS). -5% & 6% up to 10% (GS) in MDBK and Vero cells respectively. 0.1% EPL could be used with lower % Goat serum to enhance cellular morphology.

References

18 Cites in Article

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

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Not applicable for this article.

How to Cite This Article

Safaa A. Warda. 2019. \u201cAlternatives to Fetal Bovine Serum in Different Cell Cultures\u201d. Global Journal of Medical Research - E: Gynecology & Obstetrics GJMR-E Volume 19 (GJMR Volume 19 Issue E3): .

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GJMR Volume 19 Issue E3
Pg. 9- 16

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-E Classification: NLMC Code: WQ 211

Submission ReceivedDecember 8, 2018
Peer Review Double Blind
Handling Editor
Accepted January 4, 2019
Published January 15, 2019

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v1.2

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September 4, 2019

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