Alternatives to Fetal Bovine Serum in Different Cell Cultures

Alternatives to Fetal Bovine Serum in Different Cell Cultures

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Abstract

Culture of animal cells is a key operation in bioscience at which fetal bovine serum is the most widely used. Other new alternatives were examined for growth of cell culture as newly born calf serum (NBCS), goat serum (GS) and Equine platelet lysate (EPL). Cell growth and viability was investigated in target cells (Vero-MDBK) using different concentration of alternatives. In general the platelet lysate medium 7 -10 % (EPL) supported cell growth and maintained viabilities comparable or superior to (NBCS). Goat serum exihbit heterologous cells in the first passage then the growth were similar to newly born calf serum with adding low concentration % of (GS). -5% & 6% up to 10% (GS) in MDBK and Vero cells respectively. 0.1% EPL could be used with lower % Goat serum to enhance cellular morphology.

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Safaa A. Warda, Hossam A. Fahmy, Fatma F. Warda. 2019. "Alternatives to Fetal Bovine Serum in Different Cell Cultures". Global Journal of Medical Research - E: Gynecology & Obstetrics GJMR-E Volume 19 (GJMR Volume 19 Issue E3).

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords
Classification
GJMR-E Classification NLMC Code: WQ 211
Version of record

v1.2

Issue date
September 4, 2019

Language
English
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Alternatives to Fetal Bovine Serum in Different Cell Cultures

Safaa Warda
Safaa Warda
Hossam Fahmy
Hossam Fahmy
Fatma Warda
Fatma Warda