An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7

An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7

Article ID

87Q6Q

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An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7

Sabrina Shameen Alam University of Chittagong

Laila Khaleda

Mohammad Al-Forkan

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Abstract

An efficient in vitro regeneration system for Tori (Brassica campestris) -7 was developed from calli of hypocotyls and cotyledonary leaves. The optimum medium for callus induction was found with 0.5 mgl-1 2, 4- dichlorophenoxyacetic acid (2,4-D). The best shooting medium contained 3.0 mgl-1 6, benzyl amino purine (BAP), 0.1 mgl-1 naphtalene acetic acid (NAA), and 5.0 mgl-1 AgNO3. Maximum number of shoots were produced when 0.5 mgl- 1 kinetin was used, whereas the combined effect of 2.0 mgl-1 BAP, 0.1 mgl-1 NAA, and 5.0 mgl-1 AgNO3 regenerated calli the most. The effect of AgNO3 was found for callogenesis at 0.5 mgl-1 and for regeneration at 5.0 mgl-1. In vitro regenerated shoots of Tori-7 developed roots on medium with 1.0 mgl-1 indole 3- butyric acid (IBA). The developed efficient in vitro regeneration protocol can be used as a baseline for Agrobacteriummediated genetic transformation of the studied plants.

An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7

Authors

Sabrina Shameen Alam University of Chittagong

Laila Khaleda

Mohammad Al-Forkan

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How to Cite

Sabrina Shameen Alam. 2013. “. Global Journal of Science Frontier Research – G: Bio-Tech & Genetics GJSFR-G Volume 13 (GJSFR Volume 13 Issue G2): .

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

GJSFR Volume 13 Issue G2
Pg. 31- 34

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Submission Received September 7, 2013
Accepted September 27, 2013
Published October 7, 2013

Keywords

agrobacterium and tori-7 in vitro regeneration

Article Matrices

Total Score: 103

Country: Bangladesh

Subject: Global Journal of Science Frontier Research - G: Bio-Tech & Genetics

Authors: Sabrina Shameen Alam, Laila Khaleda, Mohammad Al-Forkan (PhD/Dr. count: 0)

View Count (all-time): 106

Total Views (Real + Logic): 4813

Total Downloads (simulated): 2359

Publish Date: 2013 10, Wed

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Total Views: 4813

Total Downloads: 2359

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